Chu Chengxiang, Wang Xianfeng, Deng Yuanyi, Ma Yi, Zou Chengyue, Yang Mei, Luo Huibo, Huo Danqun, Hou Changjun
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, PR China.
Liquor Making Biology Technology and Application of Key Laboratory of Sichuan Province, College of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 May 5;292:122380. doi: 10.1016/j.saa.2023.122380. Epub 2023 Jan 20.
A facile and rapid fluorescence sensor array based on Tb (III) and Eu (III) doped Zr (IV) metal-organic frameworks was proposed for Chinese green tea discrimination. According to large porosity of Tb@UiO-66-(COOH) and Eu@UiO-66-(COOH), phenolic hydroxyl groups of tea polyphenols could coordinate with free carboxylic acid groups and was captured into the pores, which led to the disturbance of electronic structure of ligand and inhibited the energy transfer efficiency from ligand to Tb (III) and Eu (III) center, causing the fluorescence quenching effect. Based on Hierarchy Cluster Analysis and Linear Discrimination Analysis, the fluorescence sensor array was employed for successful tea polyphenols classification through the analysis of different fluorescence quenching effect to tea polyphenols. Green tea samples within different categories and grades were also successfully discriminated using this assay according to tea polyphenols, providing a new method for Chinese green tea identification.
提出了一种基于铽（III）和铕（III）掺杂的锆（IV）金属有机框架的简便快速荧光传感器阵列用于中国绿茶鉴别。由于Tb@UiO-66-(COOH)和Eu@UiO-66-(COOH)的大孔隙率，茶多酚的酚羟基可与游离羧酸基团配位并被捕获到孔中，这导致配体电子结构的扰动并抑制了从配体到铽（III）和铕（III）中心的能量转移效率，从而产生荧光猝灭效应。基于层次聚类分析和线性判别分析，通过分析对茶多酚的不同荧光猝灭效应，该荧光传感器阵列成功用于茶多酚分类。根据茶多酚，不同类别和等级的绿茶样品也使用该方法成功鉴别，为中国绿茶鉴别提供了一种新方法。
