Wei Dandan, Huang Yulin, Liang Min, Ren Pengfei, Tao Yinghua, Xu Li, Zhang Tianzhu, Ji Zhenling, Zhang Qianli
State Key Lab of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Department of General Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
Colloids Surf B Biointerfaces. 2023 Mar;223:113159. doi: 10.1016/j.colsurfb.2023.113159. Epub 2023 Jan 20.
Polypropylene (PP) mesh has been widely used in hernia repair as prosthesis material owing to its excellent balanced biocompatibility and mechanical properties. However, abdominal adhesion between the visceral and PP mesh is still a major problem. Therefore, anti-adhesive PP mesh was designed with poly(vinyl alcohol) (PVA) hydrogel and liposomes drug delivery system. First, PVA hydrogel coating was formed on the surface of PP mesh with freezing-thawing processing cycles (FTP). Subsequently, the lyophilized PVA-c-PP was immersed in rapamycin (RPM)-loaded liposome solution until swelling equilibrated to obtain the anti-adhesion mesh RPM@LPS/PVA-c-PP. It was demonstrated that the hydrogel coating can stably fix on the surface of PP mesh even after immersed in PBS solution at 37 °C or 40 °C for up to 30 days. In vitro cell tests revealed the excellent cytocompatibility and the potential to inhibit cell adhesion of the modified PP mesh. Moreover, the anti-adhesive effects of the RPM@LPS/PVA-c-PP mesh was evaluated through in vivo experiments. The RPM@LPS/PVA-c-PP mesh exhibited less adhesion than original PP mesh throughout the duration of implantation. At 30 days, the adhesion score of RPM@LPS/PVA-c-PP mesh was 1.37 ± 0.75, however the original PP was 3 ± 0.71. Furthermore, the results of H&E and Masson trichrome staining proved that the RPM@LPS/PVA-c-PP mesh showed slighter inflammation response and significant looser fibrous tissue surrounded the PP filaments as compared to the native PP. The current findings manifested that this type of RPM@LPS/PVA-c-PP might be a potential candidate for anti-adhesion treatment. DATA AVAILABILITY: Data will be made available on request.
聚丙烯（PP）网片因其优异的平衡生物相容性和机械性能，已被广泛用作疝修补的假体材料。然而，内脏与PP网片之间的腹部粘连仍然是一个主要问题。因此，利用聚乙烯醇（PVA）水凝胶和脂质体药物递送系统设计了抗粘连PP网片。首先，通过冻融处理循环（FTP）在PP网片表面形成PVA水凝胶涂层。随后，将冻干的PVA-c-PP浸入负载雷帕霉素（RPM）的脂质体溶液中，直至溶胀平衡，以获得抗粘连网片RPM@LPS/PVA-c-PP。结果表明，即使在37℃或40℃的PBS溶液中浸泡长达30天，水凝胶涂层仍能稳定地固定在PP网片表面。体外细胞试验显示，改性PP网片具有优异的细胞相容性和抑制细胞粘附的潜力。此外，通过体内实验评估了RPM@LPS/PVA-c-PP网片的抗粘连效果。在整个植入期间，RPM@LPS/PVA-c-PP网片的粘连比原始PP网片少。在30天时，RPM@LPS/PVA-c-PP网片的粘连评分为1.37±0.75，而原始PP网片为3±0.71。此外，苏木精-伊红（H&E）和Masson三色染色结果证明，与天然PP相比，RPM@LPS/PVA-c-PP网片的炎症反应更轻，PP细丝周围的纤维组织明显更疏松。目前的研究结果表明，这种类型的RPM@LPS/PVA-c-PP可能是抗粘连治疗的潜在候选材料。数据可用性：数据将根据要求提供。
