Hu Bing, Ma Fengguang, Yang Yingkang, Xie Minhao, Zhang Chen, Xu Ye, Zeng Xiaoxiong
College of Food Science and Technology, Nanjing Agricultural University , Nanjing 210095, China.
J Agric Food Chem. 2016 May 4;64(17):3422-9. doi: 10.1021/acs.jafc.6b00931. Epub 2016 Apr 19.
Modification of chitosan (CS) through grafting with caffeic acid (CA, CA-g-CS) and ferulic acid (FA, FA-g-CS) significantly improved its solubility under neutral and alkaline environments. Spherical and physicochemically stable nanocomplexes assembled from the phenolic acid grafting CS and caseinophosphopeptide (CPP) were obtained with particle size <300 nm and zeta potential of <+30 mV. The net polymer nanocomplexes composed with the phenolic acid grafting CS and CPP showed strong antioxidant activity. The encapsulation efficiencies of epigallocatechin-3-gallate (EGCG) in the CA-g-CS-CPP nanocomplexes and FA-g-CS-CPP nanocomplexes were 88.1 ± 6.7 and 90.4 ± 4.2%, respectively. Improved delivery properties of EGCG were achieved after loading with the antioxidant nanocomplexes, including controlling release of EGCG under simulated gastric environments and preventing its degradation under neutral and alkaline environments.
通过与咖啡酸（CA，CA-g-CS）和阿魏酸（FA，FA-g-CS）接枝对壳聚糖（CS）进行改性，显著提高了其在中性和碱性环境下的溶解度。由酚酸接枝的CS和酪蛋白磷酸肽（CPP）组装而成的球形且物理化学性质稳定的纳米复合物，其粒径<300 nm，zeta电位<+30 mV。由酚酸接枝的CS和CPP组成的净聚合物纳米复合物表现出较强的抗氧化活性。表没食子儿茶素-3-没食子酸酯（EGCG）在CA-g-CS-CPP纳米复合物和FA-g-CS-CPP纳米复合物中的包封率分别为88.1±6.7%和90.4±4.2%。用抗氧化纳米复合物负载后，EGCG的递送性能得到改善，包括在模拟胃环境中控制EGCG的释放以及在中性和碱性环境中防止其降解。
