State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China.
Food Chem. 2017 Sep 15;231:19-24. doi: 10.1016/j.foodchem.2017.02.106. Epub 2017 Feb 22.
The objective of this study was to prepare chitosan nanoparticles (CS NPs) coated with zein as a promising encapsulation and delivery system for epigallocatechin gallate (EGCG). The factors influencing the nanoparticle fabrication, including zein concentration, zein/chitosan weight ratio and EGCG encapsulation percentage, were systematically investigated. The physicochemical and structural analysis showed that the electrostatic interactions and hydrogen bonds were the major forces responsible for nanoparticles formation. The transmission electron microscopy study revealed the spherical nature with smooth surface of obtained nanoparticles. The release profile of EGCG showed a burst effect, followed by slow release. EGCG release was relatively higher from zein/chitosan nanoparticles (zein/CS NPs) with higher DPPH scavenging activity, than that of NPs without zein coating in 95% ethanol fatty simulant. These results indicated that controlled-release of EGCG from zein/CS NPs and its corresponding antioxidant activities in 95% ethanol fatty simulant may provide long-term protection against oxidation for fatty foods.
本研究旨在制备壳聚糖纳米粒子（CS NPs），并用玉米醇溶蛋白进行涂层，作为没食子儿茶素没食子酸酯（EGCG）的一种有前途的封装和递送系统。系统研究了影响纳米颗粒制备的因素，包括玉米醇溶蛋白浓度、玉米醇溶蛋白/壳聚糖的重量比和 EGCG 的包封率。物理化学和结构分析表明，静电相互作用和氢键是导致纳米颗粒形成的主要作用力。透射电子显微镜研究表明，所得纳米颗粒具有球形，表面光滑。EGCG 的释放曲线呈现出突释效应，随后是缓慢释放。在 95%乙醇脂肪模拟物中，具有更高 DPPH 清除活性的玉米醇溶蛋白/壳聚糖纳米颗粒（zein/CS NPs）中 EGCG 的释放率高于没有玉米醇溶蛋白涂层的 NPs。这些结果表明，EGCG 从玉米醇溶蛋白/壳聚糖纳米颗粒中的控制释放及其在 95%乙醇脂肪模拟物中的相应抗氧化活性，可为脂肪类食物提供长期的抗氧化保护。
