Carbohydrate Signalling Group, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland; Advanced Glycoscience Research Cluster, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland.
Nestlé Development Centre Nutrition, Askeaton, Co. Limerick, Ireland.
Food Res Int. 2023 Feb;164:112416. doi: 10.1016/j.foodres.2022.112416. Epub 2022 Dec 27.
Human milk fat globule membrane (MFGM) and whey proteins are nutritionally and functionally valuable, with many beneficial bioactivities associated with their glycosylation. However glycosylation of milk components other than free milk oligosaccharides are underinvestigated. Whey protein concentrate (WPC) ingredients with various enrichments or depletions are used in infant formula (IF) formulations to contribute to human milk equivalence and bioactivity benefits, but their overall or global glycosylation has not been compared. We compared the global glycosylation of commercial WPC ingredients for use in various IF formulations; two MFGM-enriched WPC ingredients (high fat HF1 and lower fat HF2), an α-lactalbumin-enriched WPC (WPC Lac) which has α-lactalbumin concentration closer to human milk and significantly less β-lactoglobulin which is not present in human milk, and two base WPC ingredients (WPC 80 and WPC 35) using lectin microarray profiling. WPC Lac and WPC HF1 glycosylation were highly similar to each other and both somewhat similar to WPC 35, while WPC HF2 was more similar to the base WPC 80 ingredient. N-linked glycosylation analysis demonstrated that WPC HF1 and WPC Lac were qualitatively most similar to one another, with WPC 80 and WPC 35 having similar structures, confirming lectin microarray profiling as a valuable method to compare global glycosylation. Thus WPC Lac may be a valuable ingredient for providing equivalent glycosylation to MFGM supplementation.
人乳脂肪球膜 (MFGM) 和乳清蛋白在营养和功能上都很有价值，其糖基化与许多有益的生物活性有关。然而，除了游离乳寡糖之外的其他乳成分的糖基化研究较少。在婴儿配方奶粉 (IF) 配方中使用各种浓缩或 depleted 的乳清蛋白浓缩物 (WPC) 成分，以达到与母乳等效和生物活性的益处，但它们的整体或全局糖基化尚未进行比较。我们比较了用于各种 IF 配方的商业 WPC 成分的全局糖基化；两种 MFGM 浓缩的 WPC 成分（高脂肪 HF1 和低脂肪 HF2）、一种与母乳中 α-乳白蛋白浓度更接近、β-乳球蛋白含量显著较少（β-乳球蛋白不存在于母乳中）的 α-乳白蛋白浓缩 WPC（WPC Lac），以及两种基础 WPC 成分（WPC 80 和 WPC 35），使用凝集素微阵列分析。WPC Lac 和 WPC HF1 的糖基化非常相似，与 WPC 35 也有些相似，而 WPC HF2 与基础 WPC 80 成分更相似。N-连接糖基化分析表明，WPC HF1 和 WPC Lac 在质量上彼此最为相似，WPC 80 和 WPC 35 的结构相似，证实了凝集素微阵列分析是比较全局糖基化的一种有价值的方法。因此，WPC Lac 可能是提供与 MFGM 补充等效糖基化的有价值的成分。
