Monaco Marcia H, Gross Gabriele, Donovan Sharon M
Department of Food Science & Human Nutrition, University of Illinois, Urbana, IL, United States.
Medical and Scientific Affairs, Reckitt Benckiser/Mead Johnson Nutrition Institute, Nijmegen, Netherlands.
Front Pediatr. 2021 Sep 1;9:731005. doi: 10.3389/fped.2021.731005. eCollection 2021.
The milk fat globule membrane (MFMG) is a complex milk component that has been shown to inhibit rotavirus (RV) binding to cell membranes . Herein, a whey protein lipid concentrate high in MFGM components (WPLC) and whey protein concentrate (WPC; control) were screened for anti-infective activity against porcine OSU and human Wa strains of RV in both the African Green Monkey kidney (MA104) and the human colorectal adenocarcinoma (Caco-2) cell lines. Confluent cells were exposed to OSU or Wa RV in the presence of WPLC or WPC (control) at 0, 0.1, 0.5, 1.0, 2.5, or 5 mg/ml. Infectivity was detected by immunohistochemistry and expressed as % inhibition relative to 0 mg/ml. WPLC efficacy over WPC was expressed as fold-change. One-way ANOVA analyzed data for the independent and interactive effects of concentration, test material, and RV strain. Both WPLC and WPC exhibited concentration-dependent inhibition of human Wa and porcine OSU RV infectivity in MA104 and Caco-2 cells ( < 0.0001). WPLC was 1.5-4.8-fold more effective in reducing infectivity than WPC. WPLC efficacy was independent of RV strains, but varied between cell lines. WPLC and WPC at concentrations ≥0.5 mg/mL were most effective in reducing human Wa RV infectivity in MA104 cells ( < 0.0001). WPLC decreased infectivity of two strains for RV which differ in their dependency on sialic acid for binding to cells. Inhibition was observed in the most commonly used cell type for RV infectivity assays (MA104) and an intestinal cell line (Caco-2). An effect on virus infectivity might be a potential mechanisms of action contributing to beneficial effects of supplementation of infant formula with MGFM reducing the risk of infections and consequently diarrhea incidence in infants.
乳脂肪球膜（MFMG）是一种复杂的乳成分，已被证明能抑制轮状病毒（RV）与细胞膜的结合。在此，对富含乳脂肪球膜成分的乳清蛋白脂质浓缩物（WPLC）和乳清蛋白浓缩物（WPC；对照）进行了筛选，以检测其对非洲绿猴肾（MA104）和人结肠腺癌（Caco-2）细胞系中猪OSU株和人Wa株RV的抗感染活性。将汇合的细胞在存在WPLC或WPC（对照）的情况下，以0、0.1、0.5、1.0、2.5或5 mg/ml的浓度暴露于OSU或Wa RV。通过免疫组织化学检测感染性，并表示为相对于0 mg/ml的抑制百分比。WPLC相对于WPC的功效表示为变化倍数。单向方差分析分析了浓度、测试材料和RV株的独立和交互作用的数据。WPLC和WPC在MA104和Caco-2细胞中均表现出对人Wa和猪OSU RV感染性的浓度依赖性抑制（<0.0001）。WPLC在降低感染性方面比WPC有效1.5至4.8倍。WPLC的功效与RV株无关，但在不同细胞系之间有所不同。浓度≥0.5 mg/mL的WPLC和WPC在降低MA104细胞中人Wa RV感染性方面最有效（<0.0001）。WPLC降低了两种对唾液酸结合细胞依赖性不同的RV株的感染性。在RV感染性检测中最常用的细胞类型（MA104）和肠道细胞系（Caco-2）中均观察到抑制作用。对病毒感染性的影响可能是一种潜在的作用机制，有助于解释在婴儿配方奶粉中添加MGFM降低感染风险并因此降低婴儿腹泻发病率的有益效果。
