Department of Applied Chemistry, Cochin University of Science and Technology, Kochi, 682022, Kerala, India.
Department of Applied Chemistry, Cochin University of Science and Technology, Kochi, 682022, Kerala, India.
Talanta. 2023 May 1;256:124321. doi: 10.1016/j.talanta.2023.124321. Epub 2023 Jan 31.
Tryptophan capped gold-silver bimetallic nanoclusters (Trp-Au/Ag BNCs) based turn-off fluorescence sensor has been developed for the simple and rapid determination of histamine. Sensor parameters such as the effect of solvent, irradiation time etc. were optimized and further studies were done using these results. Fluorescence quenching of BNCs happens with the incremental addition of histamine. This might be due to the aggregation of BNCs induced by the electrostatic force of attraction between the negatively charged BNCs and amino functional groups of histamine. The sensor showed good sensitivity for the determination of histamine with a linear range of 8.5 × 10 M to 4.0 × 10 M and a limit of detection (LOD) of 9.0 × 10 M. Practical utility of the proposed sensor has been proved through spike recovery analysis in synthetic physiological fluids.
色氨酸封端的金银双金属纳米团簇（Trp-Au/Ag BNCs）作为关闭型荧光传感器已被开发用于简单、快速地测定组氨酸。优化了传感器参数，如溶剂的影响、辐照时间等，并进一步利用这些结果进行了研究。随着组氨酸的逐渐加入，BNCs 的荧光猝灭。这可能是由于带负电荷的 BNCs 和组氨酸的氨基官能团之间的静电力引起的 BNCs 的聚集。该传感器对组氨酸的测定具有良好的灵敏度，线性范围为 8.5×10-7 M 至 4.0×10-6 M，检测限（LOD）为 9.0×10-7 M。通过在合成生理溶液中的加标回收分析证明了所提出的传感器的实际应用。
