Universidad Nacional de Colombia-Sede Bogotá, Faculty of Sciences, Department of Pharmacy, GIFFUN, Bogotá, 111321, Colombia.
Royal Botanic Gardens Kew, Richmond, Surrey, London, TW9 3AB, United Kingdom.
J Ethnopharmacol. 2023 May 10;307:116218. doi: 10.1016/j.jep.2023.116218. Epub 2023 Feb 2.
Neuronal death is a central process in neurodegenerative diseases and represents a therapeutic challenge for their prevention and treatment. Scutellaria incarnata Vent. roots are used traditionally in Colombia for central nervous system conditions including those affecting cognitive functions, but their chemistry and neuroprotective action remain to be explored to understand the scientific basis for their medicinal uses. In this study, S. incarnata roots are investigated to assess whether they have neuroprotective effects that could provide some explanation for their traditional use in neurodegenerative diseases.
To evaluate the neuroprotective effect of S. incarnata roots and its chemical constituents against C-ceramide-induced cell death in Cath.-a-differentiated (CAD) cells.
S. incarnata root ethanol extract was fractionated and compounds were isolated by column chromatography; their structures were elucidated by nuclear magnetic resonance spectroscopy, mass spectrometry and infrared spectroscopy. The cytotoxic and neuroprotective effects against C-ceramide of S. incarnata root extract, fractions and isolated compounds were assessed in CAD cells.
S. incarnata root extract and its n-butanol fraction were not cytotoxic but showed neuroprotective effects against C-ceramide toxicity in CAD cells. The phenylethanoid glycosides incarnatoside (isolated for the first time) and stachysoside C (12.5, 25 and 50 μg/mL) from S. incarnata roots also protected CAD cells against C-ceramide without inducing cytotoxic effects.
The observed neuroprotective effects of S. incarnata root extract and isolated phenylethanoid glycosides in CAD cells provide an ethnopharmacological basis for the traditional use of this species in Colombia for central nervous system disorders.
神经元死亡是神经退行性疾病的核心过程，代表了预防和治疗这些疾病的治疗挑战。黄芩根在哥伦比亚传统上用于治疗中枢神经系统疾病，包括影响认知功能的疾病，但它们的化学成分和神经保护作用仍有待探索，以了解其药用的科学依据。在这项研究中，研究了黄芩根，以评估其是否具有神经保护作用，可以为其在神经退行性疾病中的传统用途提供一些解释。
评估黄芩根及其化学成分对 C-神经酰胺诱导的 CAD 细胞死亡的神经保护作用。
黄芩根乙醇提取物经柱层析分离得到化合物；通过核磁共振波谱、质谱和红外光谱对其结构进行了阐明。用 CAD 细胞评估黄芩根提取物、馏分和分离化合物对 C-神经酰胺的细胞毒性和神经保护作用。
黄芩根提取物及其正丁醇馏分无细胞毒性，但对 CAD 细胞中 C-神经酰胺毒性具有神经保护作用。从黄芩根中分离得到的苯乙醇苷类化合物 incarnatoside（首次分离得到）和 stachysoside C（12.5、25 和 50μg/mL）也能保护 CAD 细胞免受 C-神经酰胺的毒性作用，而不引起细胞毒性。
黄芩根提取物和分离得到的苯乙醇苷类化合物在 CAD 细胞中的观察到的神经保护作用为该物种在哥伦比亚用于治疗中枢神经系统疾病的传统用途提供了民族药理学基础。
