Niimi T, Imaizumi M, Abe T, Haimoto H, Nagura H
Department of Thoracic Surgery, Nagoya University School of Medicine, Japan.
Am J Clin Pathol. 1987 Nov;88(5):545-51. doi: 10.1093/ajcp/88.5.545.
To elucidate the characteristics of metaplastic changes of bronchial mucosa, the distribution of four epithelial antigens and two subtypes of enolases was studied immunohistochemically. The authors classified the metaplastic changes into three types: basal cell hyperplasia, stratification, and squamous metaplasia. Secretory component (SC) was detected in all lesions with stratification and in three of ten lesions of squamous metaplasia. Epithelial membrane antigen (EMA) was localized not only on the luminal surfaces but also among the stratified layers of metaplastic epithelium. No carcinoembryonic antigen (CEA) was detectable in normal epithelium, whereas almost all metaplastic lesions had both nonspecific cross-reacting antigen (NCA) and CEA immunoreactivity. In normal mucosa, basal cells were strongly positive for alpha-enolase but negative for gamma-enolase, and columnar cells expressed both enolases. In areas of metaplasia, alpha-enolase was present throughout the layer, but gamma-enolase was absent. These immunohistochemical findings suggest that metaplastic squamous epithelial cells have glandular differentiation and that several biochemical and metabolic aberrations occur during the process of metaplasia.
为阐明支气管黏膜化生改变的特征，采用免疫组织化学方法研究了四种上皮抗原和两种烯醇化酶亚型的分布。作者将化生改变分为三种类型：基底细胞增生、分层和鳞状化生。在所有分层病变及十例鳞状化生病变中的三例中检测到分泌成分（SC）。上皮膜抗原（EMA）不仅定位于管腔表面，也定位于化生上皮的分层中。在正常上皮中未检测到癌胚抗原（CEA），而几乎所有化生病变均有非特异性交叉反应抗原（NCA）和CEA免疫反应性。在正常黏膜中，基底细胞α烯醇化酶呈强阳性，γ烯醇化酶呈阴性，柱状细胞同时表达两种烯醇化酶。在化生区域，α烯醇化酶存在于全层，但γ烯醇化酶缺失。这些免疫组织化学结果表明，化生的鳞状上皮细胞具有腺性分化，并且在化生过程中发生了一些生化和代谢异常。
