Moses G C, Lightle G O, Tuckerman J F, Henderson A R
Department of Clinical Biochemistry, University Hospital, London, Ontario, Canada.
Am J Clin Pathol. 1987 Nov;88(5):603-9. doi: 10.1093/ajcp/88.5.603.
The authors evaluated the Cobas FARA centrifugal analyzer with respect to pipetting precision and accuracy, instrument temperature, spectrophotometric response, and analytic performance for the assay of five serum enzymes and glucose. Spectrophotometric response, temperature response, pipetting precision, and accuracy were satisfactory. However, sufficient time must be allowed for cuvet contents to reach a stable temperature before measurements are made. Total day-to-day imprecision (within plus between run) was less than 5% (coefficient of variation) for aspartate and alanine aminotransferases (AST; Enzyme Commission classification number [EC] EC 2.6.1.1; and ALT; EC 2.6.1.2); alkaline phosphatase (AP; EC 3.1.3.1); gamma-glutamyltransferase (GGT; EC 2.3.1.2); lactate dehydrogenase (LD; EC 1.1.1.17); creatine kinase (CK; EC 2.7.3.1); and glucose assays. Results compare well with those obtained with other current clinical chemistry analyzers; correlation coefficients were greater than 0.993. Sample-to-sample carryover was negligible, and method linearity was satisfactory for all tests.
作者对Cobas FARA离心分析仪在移液精度和准确性、仪器温度、分光光度响应以及五种血清酶和葡萄糖检测的分析性能方面进行了评估。分光光度响应、温度响应、移液精度和准确性均令人满意。然而，在进行测量之前，必须留出足够的时间使比色皿中的内容物达到稳定温度。天冬氨酸氨基转移酶（AST；酶委员会分类编号[EC]EC 2.6.1.1）、丙氨酸氨基转移酶（ALT；EC 2.6.1.2）、碱性磷酸酶（AP；EC 3.1.3.1）、γ-谷氨酰转移酶（GGT；EC 2.3.1.2）、乳酸脱氢酶（LD；EC 1.1.1.17）、肌酸激酶（CK；EC 2.7.3.1）和葡萄糖检测的总日间不精密度（批内加批间）小于5%（变异系数）。结果与其他当前临床化学分析仪获得的结果相比良好；相关系数大于0.993。样品间的携带污染可忽略不计，所有检测的方法线性均令人满意。
