Gerontology Research Center and Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.
Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.
J Extracell Vesicles. 2023 Feb;12(2):e12308. doi: 10.1002/jev2.12308.
Decreased systemic oestrogen levels (i.e., menopause) affect metabolic health. However, the detailed mechanisms underlying this process remain unclear. Both oestrogens and exercise have been shown to improve metabolic health, which may be partly mediated by circulating microRNA (c-miR) signalling. In recent years, extracellular vesicles (EV) have increased interest in the field of tissue crosstalk. However, in many studies on EV-carried miRs, the co-isolation of high-density lipoprotein (HDL) particles with EVs has not been considered, potentially affecting the results. Here, we demonstrate that EV and HDL particles have distinct small RNA (sRNA) content, including both host and nonhost sRNAs. Exercise caused an acute increase in relative miR abundancy in EVs, whereas in HDL particles, it caused an increase in transfer RNA-derived sRNA. Furthermore, we demonstrate that oestrogen-based hormonal therapy (HT) allows the acute exercise-induced miR-response to occur in both EV and HDL particles in postmenopausal women, while the response was absent in nonusers.
雌激素水平降低（即绝经）会影响代谢健康。然而，这一过程的详细机制仍不清楚。雌激素和运动都已被证明可以改善代谢健康，这可能部分是通过循环 microRNA (c-miR) 信号介导的。近年来，细胞外囊泡 (EV) 在组织串扰领域引起了越来越多的关注。然而，在许多关于 EV 携带的 miR 的研究中，并没有考虑到与 EV 共分离的高密度脂蛋白 (HDL) 颗粒，这可能会影响结果。在这里，我们证明 EV 和 HDL 颗粒具有不同的小 RNA (sRNA) 含量，包括宿主和非宿主 sRNAs。运动导致 EV 中相对 miR 丰度的急性增加，而在 HDL 颗粒中，运动导致转移 RNA 衍生的 sRNA 增加。此外，我们证明基于雌激素的激素治疗 (HT) 允许绝经后妇女的 EV 和 HDL 颗粒中发生急性运动诱导的 miR 反应，而在未使用者中则不存在这种反应。
