Yisa Abraham Gana, Chia Mathias Ahii, Gadzama Ibrahim Madu Katsallah, Oniye Sonnie Joshua, Sha'aba Ramatu Idris, Gauje Balli
Department of Zoology, Ahmadu Bello University, Zaria, Nigeria.
Department of Botany, Ahmadu Bello University, Zaria, Nigeria.
Environ Toxicol Pharmacol. 2023 Mar;98:104078. doi: 10.1016/j.etap.2023.104078. Epub 2023 Feb 3.
Amoxicillin (AMX) and ciprofloxacin (CPX) are broad-spectrum antibiotics with wide application in agriculture and human and veterinary medicine. The drugs end up in the environment where their impact on zooplankton remains scantily understood. This study investigated the immobilization, risk assessment (RQ), antioxidant response, and biochemical changes of Daphnia magna post-exposure to AMX and CPX. Sixty-percent immobilization of Daphnia occurred at 200 μg L AMX and CPX, while EC values were 2391.6 μg L and 273.4 μg L, respectively. RQs were 113.3 and 11,481.5, while Toxic units were 41.6 and 364.9 for AMX and CPX, respectively. Both antibiotics caused a significant rise in intracellular hydrogen peroxide 48 h post-exposure, indicating oxidative stress. Lipid peroxidation and antioxidant enzyme activity were considerably altered during the research. Thus, environmentally relevant concentrations of AMX and CPX pose an adverse risk that could change the population dynamics of Daphnia magna.
阿莫西林（AMX）和环丙沙星（CPX）是广谱抗生素，在农业、人类医学和兽医学中有着广泛应用。这些药物最终进入环境，但其对浮游动物的影响仍知之甚少。本研究调查了大型溞暴露于AMX和CPX后的固定化、风险评估（RQ）、抗氧化反应及生化变化。当AMX和CPX浓度为200μg/L时，大型溞出现60%的固定化，而其EC值分别为2391.6μg/L和273.4μg/L。AMX和CPX的RQ分别为113.3和11481.5，毒性单位分别为41.6和364.9。两种抗生素在暴露48小时后均导致细胞内过氧化氢显著升高，表明存在氧化应激。在研究过程中，脂质过氧化和抗氧化酶活性发生了显著变化。因此，与环境相关浓度的AMX和CPX会带来不利风险，可能改变大型溞的种群动态。
