College of Sciences, Northeastern University, Shenyang 110819, China; Jihua Institute of Biomedical Engineering and Technology, Jihua Laboratory, Foshan 528200, China.
Jihua Institute of Biomedical Engineering and Technology, Jihua Laboratory, Foshan 528200, China.
Int J Biol Macromol. 2024 Oct;278(Pt 3):134943. doi: 10.1016/j.ijbiomac.2024.134943. Epub 2024 Aug 20.
Carboxymethyl chitosan (CMCS) and sodium alginate (SA), which are excellent polysaccharide-based hemostatic agents, are capable of forming polyelectrolyte complexes (PEC) through electrostatic interactions. However, CMCS/SA PEC sponges prepared by the conventional sol-gel process exhibited slow liquid absorption rate and poor mechanical properties post-swelling. In this work, a novel strategy involving freeze casting followed by acetic acid vapor treatment to induce electrostatic interactions was developed to fabricate novel PEC sponges with varying CMCS/SA mass ratios. Compared to sol-gel process sponge, the novel sponge exhibited a higher density of electrostatic interactions, resulting in denser pore walls that resist re-gelation and swelling according to FTIR, XRD, and SEM analyses. Additionally, the liquid absorption kinetics, as well as compression and tension tests, demonstrated that the novel sponge had significantly improved rapid blood absorption capacity and mechanical properties. Furthermore, in vitro coagulation and drug release studies showed that the novel sponge had a lower blood clotting index and clotting time, along with a slower drug release rate after loading with berberine hydrochloride, showcasing its potential as a rapid hemostatic dressing with controlled drug release capabilities.
羧甲基壳聚糖（CMCS）和海藻酸钠（SA）是两种出色的多糖类止血剂，它们能够通过静电相互作用形成聚电解质复合物（PEC）。然而，通过传统的溶胶-凝胶工艺制备的 CMCS/SA PEC 海绵存在液体吸收速率慢和溶胀后力学性能差的问题。在这项工作中，我们开发了一种涉及冷冻铸造和乙酸蒸气处理以诱导静电相互作用的新策略，用于制备具有不同 CMCS/SA 质量比的新型 PEC 海绵。与溶胶-凝胶工艺海绵相比，新型海绵具有更高密度的静电相互作用，根据 FTIR、XRD 和 SEM 分析，这导致了更致密的孔壁，能够抵抗再凝胶化和溶胀。此外，液体吸收动力学以及压缩和拉伸测试表明，新型海绵具有显著改善的快速血液吸收能力和力学性能。此外，体外凝血和药物释放研究表明，新型海绵具有较低的凝血指数和凝血时间，并且在负载盐酸小檗碱后具有较慢的药物释放速率，展示了其作为具有控释药物能力的快速止血敷料的潜力。
