Department of Pharmacy, The Second Affiliated Hospital, Army Medical University, 183 Xinqiao Road, Chongqing 400000, China.
Department of Pharmacy, Chongqing Emergency Medical Center, Chongqing University Central Hospital, 1 Jiankang Road, Yuzhong District, Chongqing 400014, China.
Carbohydr Polym. 2022 Oct 15;294:119805. doi: 10.1016/j.carbpol.2022.119805. Epub 2022 Jul 2.
Uncontrolled hemorrhage poses a severe life-threatening situation. However, traditional hemostats still have various limitations. It is urgent to develop a material with excellent biocompatibility and hemostatic ability. Evidence has shown that carboxymethyl chitosan (CMCS) has hemostatic properties and good compatibility. Herein, we develop an expandable hemostatic sponge by modifying CMCS with cellulose nanofibrils (CNFs) through the CO-NH cross-linking method. We verified its potential as a hemostatic agent both in vivo and in vitro. The results demonstrated that the prepared carboxymethyl chitosan/cellulose nanofiber composite (CNF-CMCS) sponges could absorb blood, quickly expand to exert pressure in the wound, and exhibit an excellent coagulation ability. The CNF-CMCS sponges significantly decreased the bleeding time and blood loss in several hemorrhage models and possessed a significant advantage in treating the deep penetrating injury hemorrhage. Therefore, the sponges provide a unique application prospect and potential as a penetrating trauma hemostatic agent.
失控性出血会导致严重的生命威胁。然而，传统的止血剂仍存在各种局限性。因此，迫切需要开发一种具有优异生物相容性和止血能力的材料。有证据表明，羧甲基壳聚糖（CMCS）具有止血性能和良好的相容性。在此，我们通过 CO-NH 交联法将纤维素纳米纤维（CNFs）改性 CMCS，开发了一种可膨胀的止血海绵。我们在体内和体外验证了其作为止血剂的潜力。结果表明，制备的羧甲基壳聚糖/纤维素纳米纤维复合材料（CNF-CMCS）海绵可以吸收血液，迅速膨胀在伤口处施加压力，并表现出优异的凝血能力。CNF-CMCS 海绵显著缩短了几种出血模型的出血时间和失血量，在治疗深部穿透性损伤出血方面具有显著优势。因此，该海绵作为一种穿透性创伤止血剂具有独特的应用前景和潜力。
