Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China.
Faculty of Chemistry and Environmental Science, Guangdong Ocean University, Zhanjiang 524088, China.
Int J Biol Macromol. 2024 Aug;274(Pt 1):133265. doi: 10.1016/j.ijbiomac.2024.133265. Epub 2024 Jun 21.
Hemostasis is the first step of emergency medical treatment. It is particularly important to develop rapid-acting and efficacious hemostatic materials. Carboxymethyl chitosan (CMCS), sodium alginate (SA) and Resina Draconis (RD) were composited uniformly by polyelectrolyte blending. Their composite sponges (CMCS/SA/RD) were prepared by freeze-induced phase separation. CMCS/SA/RD sponges were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy, and their blood absorption and hemolysis ratio were analyzed. The hemostatic effect of the composite sponges was evaluated by coagulation in vitro and in vivo. The composite sponges had a porous network structure. The water absorption ratio was >8000 %, and hemolysis ratio was <5 %. CMCS/SA/RD-II and CMCS/SA/RD-III composite sponges shortened the coagulation time in vitro by 11.33 s and 9.66 s, the hepatic hemostasis time by 13.8 % and 23.3 %, and the hemostasis time after mouse-tail amputation by 28.9 % and 23.9 %, respectively. A preliminary study on its coagulation mechanism showed that CMCS/SA/RD had significant effects on erythrocyte adsorption, platelet adhesion, and shortening of the activated partial thromboplastin time.
止血是急救治疗的第一步。开发快速起效和高效的止血材料尤为重要。羧甲基壳聚糖（CMCS）、海藻酸钠（SA）和龙血竭（RD）通过聚电解质共混均匀复合。通过冷冻诱导相分离制备它们的复合海绵（CMCS/SA/RD）。通过傅里叶变换红外光谱和扫描电子显微镜对 CMCS/SA/RD 海绵进行了表征，并分析了其血液吸收和溶血率。通过体外和体内凝血评估了复合海绵的止血效果。复合海绵具有多孔网络结构。吸水率>8000%，溶血率<5%。CMCS/SA/RD-II 和 CMCS/SA/RD-III 复合海绵分别将体外凝血时间缩短了 11.33 秒和 9.66 秒，肝止血时间缩短了 13.8%和 23.3%，小鼠尾巴切断后的止血时间缩短了 28.9%和 23.9%。对其凝血机制的初步研究表明，CMCS/SA/RD 对红细胞吸附、血小板黏附以及活化部分凝血活酶时间缩短有显著影响。
