Toll 样受体 4 与糖尿病条件下耐多药结核病的相关性表达。

Correlation expression Toll-like receptor 4 with multidrugs resistant tuberculosis in diabetes mellitus condition.

机构信息

Faculty of Medicine, Hasanuddin University, Indonesia.

出版信息

Indian J Tuberc. 2023 Jan;70(1):59-64. doi: 10.1016/j.ijtb.2022.03.012. Epub 2022 Mar 17.

DOI:10.1016/j.ijtb.2022.03.012

PMID:36740319

Abstract

BACKGROUND

Toll-like receptor (TLR) are ligand homologous protein in the APC cell membrane that has functions as a receptor to triger leukocytes and innate immune responses. When there is a Microbacterium tuberculosis (MTB) infection enters from droplets to the lungs, the alveolar macrophages perform a phagocytic function. The interaction between M. tuberculosis and the TLR macrophage receptors produces chemokines which induce migration of monocytes and dendrite cells for destruction. Diabetes militus (DM) has become risk factor for developing tuberculosis. DM condition will reduce immunity and the ability of immune cell phagocytes bactery and triger severe infections. The consequences of more severe infection and metabolic disorders that occur make a person more likely to experience Multidrugs resistant MTB. Not much data that reports on the expression of TLR4 as a ligand that triggers an immune response in conditions of MDR and DM. We try to find out correlation between TLR-4 in MDR MTB, diabetes and level of MTB bacteria in experimental animals.

METHODS

We conducted an experimental study on 30 experimental mice weighing 25 grams consisting of negative control grub, infected with MTB, infected with MDR MTB, negative control diabetes, MTB DM, MDR MTB DM. DM animals were induced by streptozosin to experience DM, then in the treatment of infection, intraperitoneal MTB and MDR MTB bacterial injections were given. Termination was carried out on day 14. We count number of bacteria level in the lungs and perform evaluation TLR4 from blood sampel.

RESULTS

The negative control group had mean TLR value of 1.47 (± 0.46) while the MTB group showed an increase in TLR 9.22 (± 0.39) followed by MDR MTB 9.50 (± 0.29), DM negative control 9, 21 (± 0.24) and more increasing in conditions of DM MTB 13.36 (± 0.32) and DM MDR MTB 13.35 (± 0.34). ANOVA analysis showed a significant difference (P = 0.00). pearson correlation analysis find strong correlation TLR4 in MTB and MDR MTB with diabetes.

CONCLUSION

there were a significant difference level TLR4 between MTB and MDR TB infection with diabetes. higher TLR4 level higher in DM MTB, DM MDR MTB. TLR 4 strong correlates with an increase in the number of MTB bacteria.

摘要

背景

Toll 样受体 (TLR) 是 APC 细胞膜上的配体同源蛋白，具有触发白细胞和固有免疫反应的受体功能。当结核分枝杆菌 (MTB) 从飞沫进入肺部感染时，肺泡巨噬细胞发挥吞噬功能。结核分枝杆菌与 TLR 巨噬细胞受体的相互作用产生趋化因子，诱导单核细胞和树突状细胞迁移以进行破坏。糖尿病 (DM) 已成为结核病发病的危险因素。DM 状况会降低免疫力和免疫细胞吞噬细菌的能力，并引发严重感染。更严重的感染和代谢紊乱的后果使人们更容易感染耐多药 MTB。关于 TLR4 作为触发免疫反应的配体在 MDR 和 DM 条件下的表达，没有多少数据报道。我们试图在实验动物中找出 MDR MTB、糖尿病和 MTB 细菌水平之间的 TLR-4 相关性。

方法

我们对 30 只体重 25 克的实验小鼠进行了一项实验研究，包括阴性对照 grub、感染 MTB、感染 MDR MTB、阴性对照糖尿病、MTB 糖尿病、MDR MTB 糖尿病。糖尿病动物通过链脲佐菌素诱导产生糖尿病，然后在感染治疗中，给予腹腔内 MTB 和 MDR MTB 细菌注射。第 14 天结束。我们计算肺部细菌水平，并从血液样本中评估 TLR4。

结果

阴性对照组的 TLR 值平均为 1.47(±0.46)，而 MTB 组 TLR 值升高至 9.22(±0.39)，其次是 MDR MTB 9.50(±0.29)，阴性对照组糖尿病 9.21(±0.24)，DM MTB 条件下升高更为明显 13.36(±0.32)和 DM MDR MTB 13.35(±0.34)。方差分析显示差异具有统计学意义(P=0.00)。Pearson 相关性分析发现 TLR4 在 MTB 和 MDR MTB 与糖尿病之间存在强相关性。