Agustin Heidy, Massi Muhammad Nasrum, Djaharuddin Irawati, Susanto Agus Dwi, Islam Andi Asadul, Hatta Mochammad, Bukhari Agussalim, Tabri Nur Ahmad, Santoso Arif, Patellongi Ilhamjaya
Doctoral Program of Medical Sciences, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Departement of Pulmonology and Respiratory Medicine, Faculty of Medicine, Indonesia University, Jakarta, Indonesia.
Ann Med Surg (Lond). 2021 Jul 27;68:102596. doi: 10.1016/j.amsu.2021.102596. eCollection 2021 Aug.
Tuberculosis (TB) remains a major global health problem, in the top 10 causes of death. As a regulator of the immune response, T-helper (Th) cells activate other lymphocytes from the immune system, such as B cells, to destroy the TB pathogen by releasing CD4 and CD8 Th cells. Diabetes mellitus (DM) is a known cause of developing active pulmonary TB. Few studies have examined the biomolecular expression affecting (MTB) and multidrug-resistant (MDR) MTB, which are associated with low immunity represented by TB in diabetes and CD4 and CD8 levels.
This animal study used a post-test control group design. We performed an experimental study using 30 BALB/c mice, each weighing 25 g. It included six experimental animal groups, of which three had a diabetes condition induced using intraperitoneal streptozotocin, and all were infected with MTB or MDR TB. We evaluated the CD4 and CD8 levels in each group and analyzed the differences.
We found a significant difference in CD4 and CD8 levels in MTB and MDR TB conditions.
This study shows that acute infection in experimental mice with MTB and MDR TB with or without diabetes had the highest levels of both CD4 and CD8 cells, which can be a sign of increased cellular immunity in a mice model.
结核病（TB）仍然是一个主要的全球健康问题，位列十大死因之中。作为免疫反应的调节因子，辅助性T（Th）细胞激活免疫系统中的其他淋巴细胞，如B细胞，通过释放CD4和CD8 Th细胞来破坏结核病原体。糖尿病（DM）是已知的导致活动性肺结核的病因。很少有研究探讨影响结核分枝杆菌（MTB）和耐多药（MDR）MTB的生物分子表达，它们与糖尿病中以结核病为代表的低免疫力以及CD4和CD8水平相关。
本动物研究采用试验后对照组设计。我们使用30只体重25克的BALB/c小鼠进行了一项实验研究。它包括六个实验动物组，其中三组通过腹腔注射链脲佐菌素诱导出糖尿病状态，并且所有组都感染了MTB或耐多药结核病。我们评估了每组中的CD4和CD8水平并分析了差异。
我们发现在MTB和耐多药结核病状态下CD4和CD8水平存在显著差异。
本研究表明，在有或没有糖尿病的情况下，实验小鼠感染MTB和耐多药结核病的急性感染中，CD4和CD8细胞水平最高，这可能是小鼠模型中细胞免疫增强的一个迹象。
