Pisterzi Paola, Chen Lanpeng, van Dijk Claire, Wevers Michiel J W, Bindels Eric J M, Raaijmakers Marc H G P
Department of Hematology, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
Hemasphere. 2023 Jan 31;7(2):e823. doi: 10.1097/HS9.0000000000000823. eCollection 2023 Feb.
Mesenchymal stem cells (MSCs) play pivotal roles in tissue (re)generation. In the murine bone marrow, they are thought to reside within the Sca-1 CD51 bone marrow stromal cell population. Here, using scRNAseq, we aimed to delineate the cellularheterogeneity of this MSC-enriched population throughout development. At the fetal stage, the MSC population is relatively homogeneous with subsets predicted to contain stem/progenitor cells, based on transcriptional modeling and marker expression. These subsets decline in relative size throughout life, with postnatal emergence of specialized clusters, including hematopoietic stem/progenitor cell (HSPC) niches. In fetal development, these stromal HSPC niches are lacking, but subsets of endothelial cells express HSPC factors, suggesting that they may provide initial niches for emerging hematopoiesis. This cellular taxonomy of the MSC population upon development is anticipated to provide a resource aiding the prospective identification of cellular subsets and molecular mechanisms driving bone marrow (re)generation.
间充质干细胞（MSCs）在组织（再）生成中发挥着关键作用。在小鼠骨髓中，它们被认为存在于Sca-1 CD51骨髓基质细胞群体中。在此，我们使用单细胞RNA测序（scRNAseq），旨在描绘整个发育过程中这个富含MSCs群体的细胞异质性。在胎儿阶段，基于转录建模和标志物表达，MSCs群体相对同质，其亚群预计包含干细胞/祖细胞。这些亚群在整个生命过程中相对大小下降，出生后出现了包括造血干细胞/祖细胞（HSPC）微环境在内的特化簇。在胎儿发育过程中，这些基质HSPC微环境并不存在，但内皮细胞亚群表达HSPC因子，这表明它们可能为新兴的造血提供初始微环境。预计这种发育过程中MSCs群体的细胞分类学将为前瞻性识别驱动骨髓（再）生成的细胞亚群和分子机制提供资源。
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