壳聚糖纳米颗粒对从孕妇分离出的细菌及美国典型培养物保藏中心7644菌株的抗菌效果评估

Evaluation of Chitosan Nanoparticle Antimicrobial Effect on Isolated Bacteria from Pregnant Women and ATCC 7644.

作者信息

Kazemi Rad Sara, Assmar Mehdi, Mirpour Mirsasan, Razavi Mohamad Reza

机构信息

Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.

Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.

出版信息

Iran J Public Health. 2022 Dec;51(12):2783-2790. doi: 10.18502/ijph.v51i12.11469.

DOI:10.18502/ijph.v51i12.11469

PMID:36742246

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9874196/

Abstract

BACKGROUND

is a gram positive, facultative intracellular bacteria and it is a causative agent of . Abortion is one the most important side effect of . Nano chitosan is widely used as nanomaterials considered due to its characteristics such as bactericidal and nontoxicity activity. The aim of this study was isolation of bacteria from pregnant women vaginal samples and evaluation of chitosan nanoparticles effects against them.

METHODS

Overall, 100 vaginal specimens were collected from pregnant women with and without a history of abortion referred to Tehran's Hospitals from Sep 2019 to Jul 2020 with questionnaires. Then, using biochemical methods, bacteria were isolated and identified. Isolated strains were confirmed using PCR and evaluation of gene, which is the main gene for identification of this bacterium. The effect of chitosan nanoparticles was evaluated in comparison with the antibiotics used based guideline on isolated bacteria by well diffusion method. and were determined for nanoparticle at the end.

RESULTS

Five strains of that were confirmed by PCR method. Moreover, a statistically significant relationship was observed between the isolated strains and the samples taken from women with a history of abortion. MIC and MBC for ATCC 7644 were 156.25 and 312.5 μg/ml and for 5 isolated strains were 78.12 and 158.25 μg/ml, respectively.

CONCLUSION

could be a causative agent of abortion in pregnant women. Concerning the results, Nano chitosan has acceptable antibacterial activity against .

摘要

背景

[细菌名称]是一种革兰氏阳性兼性细胞内细菌，是[疾病名称]的病原体。流产是[疾病名称]最重要的副作用之一。纳米壳聚糖因其具有杀菌和无毒等特性而被广泛用作纳米材料。本研究的目的是从孕妇阴道样本中分离[细菌名称]，并评估壳聚糖纳米颗粒对其的影响。

方法

总体而言，2019年9月至2020年7月期间，通过问卷调查从转诊至德黑兰医院的有或无流产史的孕妇中收集了100份阴道标本。然后，使用生化方法分离和鉴定[细菌名称]。使用PCR和评估[细菌主要鉴定基因名称]基因对分离出的[细菌名称]菌株进行确认。通过琼脂扩散法，将壳聚糖纳米颗粒与基于指南用于分离细菌的抗生素进行比较，评估壳聚糖纳米颗粒的效果。最后测定纳米颗粒的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。