光活化孟加拉玫瑰红功能化壳聚糖纳米颗粒具有抗菌/生物膜活性并能稳定牙本质胶原蛋白。
Photoactivated rose bengal functionalized chitosan nanoparticles produce antibacterial/biofilm activity and stabilize dentin-collagen.
作者信息
Shrestha Annie, Hamblin Michael R, Kishen Anil
机构信息
Discipline of Endodontics, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA; Department of Dermatology, Harvard Medical School, Boston, MA, USA; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, USA.
出版信息
Nanomedicine. 2014 Apr;10(3):491-501. doi: 10.1016/j.nano.2013.10.010. Epub 2013 Nov 4.
UNLABELLED
Treatment of infected teeth presents two major challenges: persistence of the bacterial-biofilm within root canals after treatment and compromised structural integrity of the dentin hard-tissue. In this study bioactive polymeric chitosan nanoparticles functionalized with rose-bengal, CSRBnp were developed to produce antibiofilm effects as well as stabilize structural-integrity by photocrosslinking dentin-collagen. CSRBnp were less toxic to fibroblasts and had significant antibacterial activity even in the presence of bovine serum albumin. CSRBnp exerted antibacterial mechanism by adhering to bacterial cell surface, permeabilizing the membrane and lysing the cells subsequent to photodynamic treatment. Photoactivated CSRBnp resulted in reduced viability of Enterococcus faecalis biofilms and disruption of biofilm structure. Incorporation of CSRBnp and photocrosslinking significantly improved resistance to degradation and mechanical strength of dentin-collagen (P<0.05). The functionalized chitosan nanoparticles provided a single-step treatment of infected root dentin by combining the properties of chitosan and that of photosensitizer to eliminate bacterial-biofilms and stabilize dentin-matrix.
FROM THE CLINICAL EDITOR
In this study, bioactive polymeric chitosan nanoparticles functionalized with rose-bengal (a photosensitizer), CSRBnp were developed to produce antibiofilm effects as well as stabilize structural-integrity of dental root dentin by photocrosslinking dentin-collagen, leading to efficient elimination of bacterial-biofilms and stabilization of dentin-matrix.
未标注
感染牙齿的治疗面临两大挑战：治疗后根管内细菌生物膜的持续存在以及牙本质硬组织结构完整性受损。在本研究中，开发了用孟加拉玫瑰红功能化的生物活性聚合物壳聚糖纳米颗粒（CSRBnp），以产生抗生物膜作用，并通过光交联牙本质胶原蛋白来稳定结构完整性。CSRBnp对成纤维细胞毒性较小，即使在存在牛血清白蛋白的情况下也具有显著的抗菌活性。CSRBnp通过粘附在细菌细胞表面发挥抗菌机制，在光动力治疗后使细胞膜通透性增加并裂解细胞。光激活的CSRBnp导致粪肠球菌生物膜的活力降低并破坏生物膜结构。加入CSRBnp并进行光交联可显著提高牙本质胶原蛋白的抗降解能力和机械强度（P<0.05）。功能化的壳聚糖纳米颗粒通过结合壳聚糖和光敏剂的特性，为感染的牙根牙本质提供了一步法治疗，以消除细菌生物膜并稳定牙本质基质。
临床编辑评论
在本研究中，开发了用孟加拉玫瑰红（一种光敏剂）功能化的生物活性聚合物壳聚糖纳米颗粒（CSRBnp），以产生抗生物膜作用，并通过光交联牙本质胶原蛋白来稳定牙根牙本质的结构完整性，从而有效消除细菌生物膜并稳定牙本质基质。
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