Parween Shaista, Kausar Hena, Alam Imtiyaz, Nehar Shamshun
Department of Zoology, Ranchi University, Ranchi, Jharkhand, India.
Ayu. 2021 Jan-Mar;42(1):57-66. doi: 10.4103/ayu.AYU_91_20. Epub 2022 Dec 7.
Search for an effective, feasible, and safe male contraceptive has been one of the major public health challenges. The present contraceptive methods are either permanent or impractical. Herbal methods are considered safe, and thus, their acceptability is higher than other prospective methods.
In the present study, oral administration of methanolic extract of (Lam.) Taub. flower was investigated for its potential role in the modulation of fertility in male albino rats.
Healthy male albino rats were randomly distributed into three groups, i.e., a control and two groups administered with 50 and 500 mg/kg body weight/day of methanolic extract of flower for 30, 90, and 180 days, respectively. Fertility records were maintained throughout the experimental period. At the end of experiment, animals were sacrificed and the weight of reproductive organs, sperm characteristics, and histopathology of testicular and epididymal tissues were evaluated. A 45-day withdrawal period was also investigated for parameters as described above for each group.
A 40% decline in fertility rate was evident in rats administered with 500 mg/kg of flower extract for 180 consecutive days. A significant reduction in testicular and epididymal weight was observed in these animals. Sperm count, motility, and viability were also reduced significantly in animals treated for 180 days. Histological evaluation of testicular cells indicated distortions in germ cell arrangements at various stages of spermatogenesis. Following 45 days of withdrawal, the resumption of normal functional and histological characteristics was apparent.
Based on the abnormalities present in the sperm characteristics and damages in testicular histology, it was confirmed that methanolic extract of flower contain antifertility potential.
寻找一种有效、可行且安全的男性避孕药一直是主要的公共卫生挑战之一。目前的避孕方法要么是永久性的，要么不切实际。草药方法被认为是安全的，因此，其可接受性高于其他预期方法。
在本研究中，研究了口服（拉马克）陶布花的甲醇提取物对雄性白化大鼠生育调节的潜在作用。
将健康雄性白化大鼠随机分为三组，即对照组和两组分别给予50和500mg/kg体重/天的花甲醇提取物，持续30、90和180天。在整个实验期间记录生育情况。实验结束时，处死动物，评估生殖器官重量、精子特征以及睾丸和附睾组织的组织病理学。还对每组上述参数进行了45天的撤药期研究。
连续180天给予500mg/kg花提取物的大鼠生育率明显下降40%。在这些动物中观察到睾丸和附睾重量显著降低。接受180天治疗的动物精子数量、活力和存活率也显著降低。睾丸细胞的组织学评估表明，在精子发生的各个阶段生殖细胞排列出现扭曲。撤药45天后，正常功能和组织学特征明显恢复。
基于精子特征异常和睾丸组织学损伤证实，花甲醇提取物具有抗生育潜力。
