Department of Medical Bioscience, University of the Western Cape, Robert Sobukwe Rd., Bellville, South Africa.
Department of Medical Bioscience, University of the Western Cape, Robert Sobukwe Rd., Bellville, South Africa; American Centre for Reproductive Medicine, Cleveland Clinic, Carnegie Ave, Cleveland, OH, USA.
J Ethnopharmacol. 2019 Sep 15;241:111972. doi: 10.1016/j.jep.2019.111972. Epub 2019 May 23.
Traditional healers use Carica papaya seeds as a remedy for diseases and as a contraceptive for men and abortion in women.
Semen samples from 35 healthy men were allowed to liquefy and subsequently incubated for 60 min in Human Tubular Fluid medium containing 1% bovine serum albumin with aqueous C. papaya seed extract at concentrations of zero, 0.025, 0.25, 2.5, 25, 250 and 2500 μg/ml. Afterwards, sperm were washed and used for assessment of capacitation and acrosome reaction, DNA fragmentation, vitality, motility, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP).
The extract showed no effects on straight-line velocity, linearity, straightness, beat-cross frequency and the percentage of capacitated, acrosome-reacted sperm. In contrast, vitality, total motility, progressive motility, curvilinear velocity, average-path velocity and the percentages of hyper-activated, ROS-positive and MMP-intact sperm decreased significantly (P < 0.05), while the percentage of DNA-fragmented sperm increased (P < 0.05).
Our data show that aqueous C. papaya seed extract significantly and negatively affects sperm motility parameters crucial for fertility; and thus, poses as a likely candidate for male contraception.
传统治疗师使用番木瓜种子作为治疗疾病的药物，并作为男性避孕药和女性堕胎药。
将 35 名健康男性的精液样本允许液化，然后在含有 1%牛血清白蛋白的人肾小管液培养基中孵育 60 分钟，培养基中含有浓度为 0、0.025、0.25、2.5、25、250 和 2500μg/ml 的番木瓜种子水提取物。之后，将精子洗涤并用于评估顶体反应、精子DNA 碎片化、活力、运动能力、活性氧 (ROS) 和线粒体膜电位 (MMP)。
提取物对直线速度、线性度、直线性、鞭打交叉频率以及顶体反应、获能精子的百分比没有影响。相比之下，精子活力、总运动力、前向运动力、曲线速度、平均路径速度以及超激活、ROS 阳性和 MMP 完整精子的百分比显著降低（P<0.05），而精子 DNA 碎片化的百分比增加（P<0.05）。
我们的数据表明，番木瓜种子水提取物显著且负面影响精子运动参数，这些参数对生育至关重要；因此，它可能是男性避孕药的候选物。
