Duan Huirong, Tiika Richard John, Tian Fuping, Lu Yuan, Zhang Qian, Hu Yu, Cui Guangxin, Yang Hongshan
Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Sciences, Lanzhou, China.
College of Forestry, Gansu Agricultural University, Lanzhou, China.
Front Plant Sci. 2023 Jan 20;13:1097076. doi: 10.3389/fpls.2022.1097076. eCollection 2022.
is one of the world's salt-tolerant plant species and is recognized as a model plant for studying the metabolism and molecular mechanisms of halophytes under salinity. To investigate the metabolic responses to salinity stress in , this study performed a widely targeted metabolomic analysis after analyzing the physiological characteristics of plants exposed to various NaCl treatments. exhibited excellent salt tolerance and could withstand extremely high NaCl concentrations, while lower NaCl conditions (50 and 100 mM) significantly promoted growth by increasing tissue succulence and maintaining a relatively stable K concentration. A total of 552 metabolites were detected, 500 of which were differently accumulated, mainly consisting of lipids, organic acids, saccharides, alcohols, amino acids, flavonoids, phenolic acids, and alkaloids. Sucrose, glucose, p-proline, quercetin and its derivatives, and kaempferol derivatives represented core metabolites that are responsive to salinity stress. Glycolysis, flavone and flavonol biosynthesis, and phenylpropanoid biosynthesis were considered as the most important pathways responsible for salt stress response by increasing the osmotic tolerance and antioxidant activities. The high accumulation of some saccharides, flavonoids, and phenolic acids under 50 mM NaCl compared with 300 mM NaCl might contribute to the improved salt tolerance under the 50 mM NaCl treatment. Furthermore, quercetin, quercetin derivatives, and kaempferol derivatives showed varied change patterns in the roots and shoots, while coumaric, caffeic, and ferulic acids increased significantly in the roots, implying that the coping strategies in the shoots and roots varied under salinity stress. These findings lay the foundation for further analysis of the mechanism underlying the response of to salinity.
是世界上耐盐植物物种之一，被公认为研究盐生植物在盐胁迫下代谢和分子机制的模式植物。为了研究其对盐胁迫的代谢响应，本研究在分析了经不同NaCl处理的植物生理特征后，进行了广泛靶向代谢组学分析。表现出优异的耐盐性，能够承受极高的NaCl浓度，而较低的NaCl条件（50和100 mM）通过增加组织多汁性和维持相对稳定的K浓度显著促进生长。共检测到552种代谢物，其中500种有不同程度的积累，主要包括脂质、有机酸、糖类、醇类、氨基酸、黄酮类、酚酸类和生物碱类。蔗糖、葡萄糖、脯氨酸、槲皮素及其衍生物和山奈酚衍生物是对盐胁迫有响应的核心代谢物。糖酵解、黄酮和黄酮醇生物合成以及苯丙烷生物合成被认为是通过提高渗透耐受性和抗氧化活性来应对盐胁迫的最重要途径。与300 mM NaCl相比，50 mM NaCl处理下一些糖类、黄酮类和酚酸类的高积累可能有助于提高50 mM NaCl处理下的耐盐性。此外，槲皮素、槲皮素衍生物和山奈酚衍生物在根和地上部表现出不同的变化模式，而香豆酸、咖啡酸和阿魏酸在根中显著增加，这意味着盐胁迫下地上部和根的应对策略不同。这些发现为进一步分析对盐胁迫响应的机制奠定了基础。
