肉鸡胃肠道中细菌DNA的有效回收及基于鸟嘌呤加胞嘧啶含量的群落结构分析
Effective recovery of bacterial DNA and percent-guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens.
作者信息
Apajalahti J H, Särkilahti L K, Mäki B R, Heikkinen J P, Nurminen P H, Holben W E
机构信息
Cultor Corporation Technology Center, FIN-02460, Kantvik, Finland.
出版信息
Appl Environ Microbiol. 1998 Oct;64(10):4084-8. doi: 10.1128/AEM.64.10.4084-4088.1998.
Abstract
A DNA-based, direct method for initial characterization of the total bacterial community in ileum and cecum of the chicken gastrointestinal (GI) tract was developed. The efficiencies of bacterial extraction and lysis were >95 and >99%, respectively, and therefore the DNA recovered should accurately reflect the bacterial communities of the ileal and cecal digesta. Total bacterial DNA samples were fractionated according to their percent G+C content. The profiles reflecting the composition of the bacterial community were reproducible within each compartment, but different between the compartments of the GI tract. This approach is independent of the culturability of the bacteria in the consortium and can be used to improve our understanding of how diet and other variables modulate the microbial communities of the GI tracts of animals.
摘要
开发了一种基于DNA的直接方法，用于初步表征鸡胃肠道（GI）回肠和盲肠中的总细菌群落。细菌提取和裂解效率分别>95%和>99%，因此回收的DNA应能准确反映回肠和盲肠消化物中的细菌群落。总细菌DNA样本根据其G+C含量百分比进行分级分离。反映细菌群落组成的图谱在每个隔室内具有可重复性，但在胃肠道的不同隔室之间有所不同。这种方法与共生体中细菌的可培养性无关，可用于增进我们对饮食和其他变量如何调节动物胃肠道微生物群落的理解。
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