Speirs J, Grierson D
Biochim Biophys Acta. 1978 Dec 21;521(2):619-33. doi: 10.1016/0005-2787(78)90303-9.
14-S RNA was purified from spinach chloroplasts. It has a molecular weight of 0.43 . 10(6) and the following nucleotide composition: 20% CMP, 23.9% AMP, 24.2% GMP and 31.9% UMP. The accumulation of 14-S RNA in chloroplasts of cotyledons of dark-grown plants is stimulated by light. Conditions are described for the isolation of 14-S RNA in the absence of appreciable fragmentation of chloroplast 23-S rRNA and the evidence that it represents a distinct type of chloroplast RNA is discussed. Translation of 14-S RNA in a protein synthesising system from Escherichia coli gives rise to two polypeptides with molecular weights of 13 200 and 12 600 and the possible role of 14-S RNA as a chloroplast messenger is discussed.
14-S RNA从菠菜叶绿体中纯化得到。其分子量为0.43×10⁶，核苷酸组成如下：20%胞嘧啶单磷酸（CMP），23.9%腺嘌呤单磷酸（AMP），24.2%鸟嘌呤单磷酸（GMP）和31.9%尿嘧啶单磷酸（UMP）。光照可刺激黑暗中生长的植物子叶叶绿体中14-S RNA的积累。文中描述了在叶绿体23-S rRNA没有明显断裂的情况下分离14-S RNA的条件，并讨论了其代表一种独特类型叶绿体RNA的证据。在大肠杆菌的蛋白质合成系统中对14-S RNA进行翻译，产生了分子量分别为13200和12600的两种多肽，并讨论了14-S RNA作为叶绿体信使的可能作用。
