Graf L, Roux E, Stutz E, Kössel H
Nucleic Acids Res. 1982 Oct 25;10(20):6369-81. doi: 10.1093/nar/10.20.6369.
The nucleotide sequence of 16S rDNA from Euglena gracilis chloroplasts has been determined representing the first complete sequence of an algal chloroplast rRNA gene. The structural part of the 16S rRNA gene has 1491 nucleotides according to a comparative analysis of our sequencing results with the published 5'- and 3'-terminal "T1-oligonucleotides" from 16S rRNA from E. gracilis. Alignment with 16S rDNA from Zea mays chloroplasts and E. coli reveals 80 to 72% sequence homology, respectively. Two deletions of 9 and 23 nucleotides are found which are identical in size and position with deletions observed in 16S rDNA of maize and tobacco chloroplasts and which seem to be characteristic for all chloroplast rRNA species. We also find insertions and deletions in E. gracilis not seen in 16S rDNA of higher plant chloroplasts. The 16S rRNA sequence of E. gracilis chloroplasts can be folded by base pairing according to the general 16S rRNA secondary structure model.
纤细裸藻叶绿体16S rDNA的核苷酸序列已被测定，这是藻类叶绿体rRNA基因的首个完整序列。根据我们的测序结果与已发表的纤细裸藻16S rRNA的5'-和3'-末端“T1-寡核苷酸”的比较分析，16S rRNA基因的结构部分有1491个核苷酸。与玉米叶绿体和大肠杆菌的16S rDNA比对显示，序列同源性分别为80%至72%。发现了两个9个和23个核苷酸的缺失，其大小和位置与玉米和烟草叶绿体16S rDNA中观察到的缺失相同，并且似乎是所有叶绿体rRNA物种的特征。我们还在纤细裸藻中发现了高等植物叶绿体16S rDNA中未见的插入和缺失。根据一般的16S rRNA二级结构模型，纤细裸藻叶绿体的16S rRNA序列可以通过碱基配对折叠。
