Endometrial microbiota in women with and without adenomyosis: A pilot study.

INTRODUCTION

The endometrial microbiota plays an essential role in the health of the female reproductive system. However, the interactions between the microbes in the endometrium and their effects on adenomyosis remain obscure.

MATERIALS AND METHODS

We profile endometrial samples from 38 women with (=21) or without (=17) adenomyosis to characterize the composition of the microbial community and its potential function in adenomyosis using 5R 16S rRNA gene sequencing.

RESULTS

The microbiota profiles of patients with adenomyosis were different from the control group without adenomyosis. Furthermore, analysis identified and as potential biomarkers for adenomyosis. In addition, , and had the most significant diagnostic value for adenomyosis. PICRUSt results identified 30 differentially regulated pathways between the two groups of patients. In particular, we found that protein export, glycolysis/gluconeogenesis, alanine, aspartate, and glutamate metabolism were upregulated in adenomyosis. Our results clarify the relationship between the endometrial microbiota and adenomyosis.

DISCUSSION

The endometrial microbiota of adenomyosis exhibits a unique structure and , and were identified as potential pathogenic microorganisms associated with adenomyosis. Our findings suggest that changes in the endometrial microbiota of patients with adenomyosis are of potential value for determining the occurrence, progression, early of diagnosis, and treatment oadenomyosis.