Institute for Systems Genetics, NYU Langone Health, New York, NY, USA.
Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York, NY, USA.
Nucleic Acids Res. 2023 Mar 21;51(5):2033-2045. doi: 10.1093/nar/gkad049.
LINE-1 retrotransposons are sequences capable of copying themselves to new genomic loci via an RNA intermediate. New studies implicate LINE-1 in a range of diseases, especially in the context of aging, but without an accurate understanding of where and when LINE-1 is expressed, a full accounting of its role in health and disease is not possible. We therefore developed a method-5' scL1seq-that makes use of a widely available library preparation method (10x Genomics 5' single cell RNA-seq) to measure LINE-1 expression in tens of thousands of single cells. We recapitulated the known pattern of LINE-1 expression in tumors-present in cancer cells, absent from immune cells-and identified hitherto undescribed LINE-1 expression in human epithelial cells and mouse hippocampal neurons. In both cases, we saw a modest increase with age, supporting recent research connecting LINE-1 to age related diseases.
LINE-1 反转录转座子是一类能够通过 RNA 中间体将自身复制到新基因组位置的序列。新的研究表明，LINE-1 与多种疾病有关，特别是在衰老的背景下，但如果不能准确了解 LINE-1 的表达位置和时间，就不可能全面了解其在健康和疾病中的作用。因此，我们开发了一种方法——5' scL1seq，该方法利用一种广泛可用的文库制备方法（10x Genomics 5' 单细胞 RNA-seq）来测量数万个单细胞中的 LINE-1 表达。我们重现了 LINE-1 在肿瘤中的已知表达模式——存在于癌细胞中，不存在于免疫细胞中——并在人类上皮细胞和小鼠海马神经元中发现了迄今为止尚未描述的 LINE-1 表达。在这两种情况下，我们都发现随着年龄的增长表达略有增加，这支持了最近将 LINE-1 与年龄相关疾病联系起来的研究。
