Division of Laboratory Animal Resources, Duke University School of Medicine, Duke University, Durham, North Carolina.
Department of Cell Biology, Duke University School of Medicine, Duke University, Durham, North Carolina.
Comp Med. 2022 Dec 1;72(6):403-409. doi: 10.30802/AALAS-CM-22-000061.
A Cancer Rainbow mouse line that expresses 3 fluorescently labeled isoforms of the tumor-driver gene (HER2BOW) was developed recently for the study of tumorigenesis in the mammary gland. The expression of 1 of the 3 HER2 isoforms in HER2BOW mice is induced through the Cre/ system. However, in addition to developing palpable mammary tumors, HER2BOW mice developed orbital tumors, specifically of the Harderian gland. Mice were euthanized, and histopathologic examination of the Harderian gland tumors was performed. Tumors were characterized by adenomatous hyperplasia to multinodular adenomas of the Harderian gland. Fluorescent imaging of the Harderian gland tissue confirmed the expression of HER2 in the tumors. Here we discuss monitoring and palliative approaches to allow attainment of humane experimental endpoints of mammary tumor growth in this mouse line. We describe a range of interventions, including close monitoring, topical palliative care, and surgical bilateral enucleation. Based on our data and previous reports in the literature, the overexpression of HER2 in Harderian gland tissue and subsequent tumor formation likely was driven by MMTV-Cre expression in the Harderian gland.
最近开发了一种表达肿瘤驱动基因 (HER2BOW) 的 3 种荧光标记同工型的癌症彩虹鼠系，用于研究乳腺肿瘤发生。HER2BOW 小鼠中 3 种 HER2 同工型之一的表达是通过 Cre/系统诱导的。然而，除了形成可触及的乳腺肿瘤外，HER2BOW 小鼠还形成了眼眶肿瘤，特别是哈德腺肿瘤。对哈德腺肿瘤进行安乐死，并进行组织病理学检查。肿瘤表现为哈德腺的腺瘤性增生至多结节腺瘤。哈德腺组织的荧光成像证实了 HER2 在肿瘤中的表达。在这里，我们讨论了监测和姑息治疗方法，以实现该小鼠系乳腺肿瘤生长的人道实验终点。我们描述了一系列干预措施，包括密切监测、局部姑息治疗和双侧眼球摘除术。基于我们的数据和文献中的先前报告，哈德腺组织中 HER2 的过表达和随后的肿瘤形成可能是由 MMTV-Cre 在哈德腺中的表达驱动的。
