Wan Pingping, Fu Huimin, Zhang Yi, Liao Cheng, Lu Qi, Xu Huajian, Mei Qingsong
School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, Anhui, China.
Department of Medical Biochemistry and Molecular Biology, School of Medicine, Jinan University, Guangzhou, 510632, Guangdong, China.
Anal Bioanal Chem. 2023 Jul;415(18):4333-4341. doi: 10.1007/s00216-023-04538-5. Epub 2023 Feb 6.
Hydrogen peroxide (HO) is considered a significant biomarker in various diseases and could induce deleterious health problems at irregular physiological concentrations. Therefore, developing a simple, efficient biocompatible nanoprobe for trace amount HO detection with high sensitivity and specificity is of great help for early diagnosis and therapeutics. Herein, we designed amphiphilic poly(styrene-co-maleic anhydride) (PMSA)-encapsulated nanoclusters composed of upconversion nanoparticles (UCNPs) and manganese dioxide nanoparticles (MnO NPs) at a specific ratio to produce a near-infrared (NIR) excited luminescent nanoprobe for HO detection. Our results revealed that the MnO NPs tended to experience catalytic decomposition when exposed to HO, while the UCNPs were retained inside the PSMA encapsulation, causing recovery of the UCNP emission band at 470 nm in accordance with HO concentration. This luminescence recovery was linearly dependent on HO concentrations, yielding a limit of detection (LOD) of 20 nM. The easy-to-interpret HO nanoprobe also proved high selectivity in the presence of other interfering substances, and biocompatibility and water-dispersibility, making it an ideal candidate for real-time detection of disease-related HO in living organisms.
过氧化氢（HO）被认为是多种疾病中的重要生物标志物，在生理浓度异常时可能引发有害的健康问题。因此，开发一种简单、高效且具有生物相容性的纳米探针，用于高灵敏度和特异性地检测痕量HO，对早期诊断和治疗有很大帮助。在此，我们设计了由上转换纳米粒子（UCNPs）和二氧化锰纳米粒子（MnO NPs）按特定比例组成的两亲性聚（苯乙烯 - 马来酸酐）（PMSA）包裹的纳米团簇，以制备用于HO检测的近红外（NIR）激发发光纳米探针。我们的结果表明，MnO NPs在暴露于HO时倾向于发生催化分解，而UCNPs则保留在PSMA包裹内部，导致470 nm处UCNP发射带根据HO浓度而恢复。这种发光恢复与HO浓度呈线性相关，检测限（LOD）为20 nM。这种易于解读的HO纳米探针在存在其他干扰物质时也表现出高选择性，以及生物相容性和水分散性，使其成为实时检测生物体内与疾病相关HO的理想候选者。
