Department of Chemistry and Chemical Engineering, Jining University, Qufu 273155, P. R. China.
School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, P. R. China.
Analyst. 2019 May 21;144(10):3347-3356. doi: 10.1039/c9an00163h. Epub 2019 Apr 12.
A new fluconazole-functionalized polyoxometalate-based metal-organic framework (POMOF) [Ag(FKZ)(HO)][HSiWO] (AgFKZSiW) was successfully constructed, and its polypyrrole (PPy) coated composite AgFKZSiW@PPy was also obtained via a facile 'in situ' oxidation polymerization process. The peroxidase-like activity evaluation indicates that the maximized synergistic effect from the integration of PPy, SiW clusters, HFKZ drug molecules, and Ag ions deeply enhanced the overall performance. More importantly, AgFKZSiW@PPy exhibits the fastest response time (30 s) among all the reported peroxidase mimics to date, including the pristine AgFKZSiW (2 min). Moreover, the AgFKZSiW@PPy-based colorimetric biosensing platform towards HO and ascorbic acid (AA) exhibits limits of detection (LOD) as low as 0.12 μM and 2.7 μM, respectively. This work reveals a promising prospect in medical diagnosis and biotechnology for colorimetric biosensor fabrication with high performance through the introduction of PPy.
一种新的氟康唑功能化多金属氧酸盐基金属有机骨架（POMOF）[Ag(FKZ)(HO)][HSiWO]（AgFKZSiW）被成功构建，并且通过简便的“原位”氧化聚合过程也获得了其聚吡咯（PPy）涂覆的复合材料 AgFKZSiW@PPy。过氧化物酶样活性评估表明，PPy、SiW 簇、HFKZ 药物分子和 Ag 离子的整合所产生的最大协同效应深刻地增强了整体性能。更重要的是，AgFKZSiW@PPy 显示出迄今为止所有报道的过氧化物酶模拟物中最快的响应时间（30 s），包括原始的 AgFKZSiW（2 min）。此外，基于 AgFKZSiW@PPy 的用于 HO 和抗坏血酸（AA）的比色生物传感平台的检测限（LOD）分别低至 0.12 μM 和 2.7 μM。通过引入 PPy，这项工作为高性能比色生物传感器的制造在医学诊断和生物技术领域揭示了一个有前途的前景。
