College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
College of Food and Biological Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Protoplasma. 2023 Jul;260(4):1179-1191. doi: 10.1007/s00709-022-01834-2. Epub 2023 Feb 6.
Mitochondria are crucial for the regulation of intracellular energy metabolism, biosynthesis, and cell survival. And studies have demonstrated the role of mitochondria in oxidative stress-induced autophagy in plants. Previous studies found that waterlogging stress can induce the opening of mitochondrial permeability transition pore (mPTP) and the release of cytochrome c in endosperm cells, which proved that mPTP plays an important role in the programmed cell death of endosperm cells under waterlogging stress. This study investigated the effects of the opening of mPTP and the inhibition of ETC on mitophagy in wheat roots under waterlogging stress. The results showed that autophagy related genes in the mitochondria of wheat root cells could respond to waterlogging stress; waterlogging stress led to the degradation of the characteristic proteins cytochrome c and COXII in the mitochondria of root cells. With the prolongation of waterlogging time, the protein degradation degree and the occurrence of mitophagy gradually increased. Under waterlogging stress, exogenous mPTP opening inhibitor CsA inhibited mitophagy in root cells and alleviated mitophagy induced by flooding stress, while exogenous mPTP opening inducer CCCP induced mitophagy in root cells; exogenous mPTP opening inducer CCCP induced mitophagy in root cells. The electron transfer chain inhibitor antimycin A induces mitophagy in wheat root cells and exacerbates mitochondrial degradation. In conclusion, waterlogging stress led to the degradation of mitochondrial characteristic proteins and the occurrence of mitophagy in wheat root cells, and the opening of mPTP and the inhibition of ETC induced the occurrence of mitophagy.
线粒体对于细胞内能量代谢、生物合成和细胞存活的调节至关重要。研究表明，线粒体在植物氧化应激诱导的自噬中起作用。先前的研究发现，水淹胁迫可以诱导胚乳细胞中线粒体通透性转换孔（mPTP）的开放和细胞色素 c 的释放，这证明 mPTP 在水淹胁迫下胚乳细胞程序性死亡中发挥重要作用。本研究探讨了水淹胁迫下 mPTP 开放和 ETC 抑制对小麦根系中自噬的影响。结果表明，水淹胁迫下小麦根细胞线粒体中的自噬相关基因能够做出响应；水淹胁迫导致根细胞线粒体中特征蛋白细胞色素 c 和 COXII 的降解。随着水淹时间的延长，蛋白降解程度和自噬的发生逐渐增加。在水淹胁迫下，外源性 mPTP 开放抑制剂 CsA 抑制了根细胞的自噬作用，缓解了水淹胁迫诱导的自噬作用，而外源性 mPTP 开放诱导剂 CCCP 则诱导了根细胞的自噬作用；外源性 mPTP 开放诱导剂 CCCP 诱导根细胞的自噬作用。电子传递链抑制剂安密妥 A 诱导小麦根细胞自噬，并加剧线粒体降解。综上所述，水淹胁迫导致小麦根细胞中线粒体特征蛋白的降解和自噬的发生，mPTP 的开放和 ETC 的抑制诱导了自噬的发生。
