Center of Reproduction, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, No. 68 Gehu Road, Jiangsu, 213003, Changzhou, China.
Laboratory of Neurological Diseases, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, 213003, Jiangsu, China.
Reprod Sci. 2023 Jul;30(7):2252-2262. doi: 10.1007/s43032-022-01158-9. Epub 2023 Feb 6.
Apolipoprotein E (ApoE) is a member of apolipoprotein (apo) family and plays critical role in lipid metabolism. In this study, the relationship between abnormal lipid metabolism caused by ApoE-deficient and male reproduction was investigated. The effect of hyperbaric oxygen (HBO) therapy on 7-month-old ApoE-knockout male mice was assessed subsequently. Mice were randomly divided into 3 groups: control group (WT), ApoE (- / -) group (AP-CON), and ApoE (- / -) plus HBO group (AP-HBO), which received HBO treatment. We found that ApoE knockout caused a decrease in male reproductive capacity due to the reduced total sperm motility, progressive motility (PR), and lower blastocyst formation rate. HBO treatment could accelerate serum lipoprotein metabolism including LDL, T-CHO, and TG and semen quality. As a result, fertilization and blastocyst formation of AP-HBO group were higher than that of AP-CON, proving positive therapeutic effect. Mechanism exploration found that HBO treatment ameliorated the testicular microenvironment by attenuating inflammatory factor production and oxidative stress, eventually improved the sperm motility. Collectively, our study provided more evidences of HBO treatment for improving the semen quality of patients with abnormal lipid metabolism caused by ApoE-deficient.
载脂蛋白 E（ApoE）是载脂蛋白（apo）家族的成员，在脂质代谢中起关键作用。本研究探讨了由 ApoE 缺乏引起的脂质代谢异常与男性生殖之间的关系。随后评估了高压氧（HBO）治疗对 7 月龄 ApoE 敲除雄性小鼠的影响。小鼠被随机分为 3 组：对照组（WT）、ApoE 缺陷组（AP-CON）和 ApoE 缺陷加 HBO 组（AP-HBO），后两组接受 HBO 治疗。我们发现 ApoE 敲除导致雄性生殖能力下降，这是由于总精子活力、前向运动精子（PR）和囊胚形成率降低所致。HBO 治疗可加速血清脂蛋白代谢，包括 LDL、总胆固醇（T-CHO）和甘油三酯（TG），以及精液质量。结果，AP-HBO 组的受精和囊胚形成率高于 AP-CON 组，证明具有积极的治疗效果。机制探索发现，HBO 治疗通过减轻炎症因子产生和氧化应激来改善睾丸微环境，最终提高精子活力。总之，本研究为 HBO 治疗改善由 ApoE 缺乏引起的脂质代谢异常患者的精液质量提供了更多证据。
