Metelev A Yu, Bogdanov A B, Ivkinl E V, Mitrokhin A A, Vodneva M M, Veliev E I
Urologiia. 2015 Sep-Oct(5):74-6.
The aim of this study was to explore the potential of hyperbaric oxygenation (HBO) for reduction of sperm DNA fragmentation level and reactive oxygen species (ROS) in semen. The study included 90 men with idiopathic infertility. Patients of the treatment group (n = 60) underwent HBO before the vitro fertilization (IVF) procedure. In the control group (n = 30) IVF was carried out without prior cours of HBO. Sperm DNA fragmentation analysis was carried out using the TUNEL assay, the level of ROS in the ejaculate was measured by chemiluminescence. HBO treatment resulted in a significant decrease in the mean level of sperm DNA fragmentation from 33.2 ± 7.5 to 11.9 ± 5.9%, and the median ROS in sperm from 0.89 to 0.39 mV/s (p < 0.05). In the control group these changes were not statistically significant. Pregnancy after IVF occurred in 63.3% (38/60) of sexual partners of the treatment group men and in 36.7% (11/30) of the control group (p < 0.05). The high efficiency of HBO in overcoming the adverse effects of oxidative stress on sperm parameters allows us to consider it as a promising method for the treatment of men with idiopathic infertility.
本研究的目的是探讨高压氧疗(HBO)降低精液中精子DNA碎片水平和活性氧(ROS)的潜力。该研究纳入了90名特发性不育男性。治疗组(n = 60)的患者在体外受精(IVF)程序前接受了高压氧疗。对照组(n = 30)在未进行高压氧疗疗程的情况下进行了体外受精。使用TUNEL测定法进行精子DNA碎片分析,通过化学发光法测量射精中的ROS水平。高压氧疗导致精子DNA碎片平均水平从33.2±7.5%显著降至11.9±5.9%,精子中ROS的中位数从0.89降至0.39 mV/s(p < 0.05)。在对照组中,这些变化无统计学意义。治疗组男性的性伴侣中,体外受精后怀孕的比例为63.3%(38/60),对照组为36.7%(11/30)(p < 0.05)。高压氧疗在克服氧化应激对精子参数的不利影响方面具有高效性,这使我们有理由将其视为治疗特发性不育男性的一种有前景的方法。