Mar B, Ekambaram M, Li K C, Zwirner J, Mei M L
Benjamin Mar, BDS, Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
Manikandan Ekambaram, BDS, MDS, PhD, Discipline of Paediatric Dentistry, Department of Oral Sciences, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
Oper Dent. 2023 Mar 1;48(2):218-225. doi: 10.2341/21-173-L.
To investigate the influence of blood and saliva contamination on the microshear bond strength (μSBS) between resin-modified glass ionomer cement (RMGIC) and resin composite (RC).
Eighty RMGIC discs were allocated into four groups (n=20). Group 1 received universal dental adhesive application in a self-etch mode followed by a build-up with RC. Group 2 received saliva as a contaminant, Group 3 received blood as a contaminant, Group 4 received a 1:1 blood-saliva mixture as a contaminant. Specimens from Groups 2, 3, and 4 were submerged into their respective contaminants for 15 seconds and dried prior to the adhesive application, followed by the protocol for Group 1. All specimens were stored in distilled water for 24 hours. Subsequently, the bonded specimens were subjected to μSBS testing using a universal testing machine. Failure mode of the debonded RMGIC surfaces was examined using scanning electron microscopy.
The μSBS from groups 1-4 were 10.76 ± 3.03 MPa, 9.36 ± 2.54 MPa, 6.55 ± 1.67 MPa and 8.42 ± 2.79 MPa, respectively. Contamination by blood and blood-saliva significantly decreased the μSBS (p<0.001, p=0.029). Saliva contamination alone had no statistically significant effect on the μSBS (p=0.524). A statistically significant difference in the mode of failure was detected between the experimental groups (p=0.012).
Saliva contamination has no influence on μSBS between RMGIC and RC when it is dried thoroughly, while blood and blood-saliva contamination reduced μSBS between RMGIC and RC even when dried thoroughly.
探讨血液和唾液污染对树脂改性玻璃离子水门汀（RMGIC）与树脂复合材料（RC）之间微剪切粘结强度（μSBS）的影响。
将80个RMGIC圆盘分为四组（n = 20）。第1组采用自酸蚀模式应用通用牙科粘结剂，然后用RC进行堆塑。第2组用唾液作为污染物，第3组用血液作为污染物，第4组用1:1的血液 - 唾液混合物作为污染物。第2、3和4组的标本在施加粘结剂之前，分别浸入各自的污染物中15秒并干燥，然后按照第1组的方案进行操作。所有标本在蒸馏水中储存24小时。随后，使用万能试验机对粘结标本进行μSBS测试。使用扫描电子显微镜检查脱粘的RMGIC表面的失效模式。
第1 - 4组的μSBS分别为10.76±3.03MPa、9.36±2.54MPa、6.55±1.67MPa和8.42±2.79MPa。血液和血液 - 唾液污染显著降低了μSBS（p<0.001，p = 0.029）。单独的唾液污染对μSBS没有统计学上的显著影响（p = 0.524）。实验组之间在失效模式上检测到统计学上的显著差异（p = 0.012）。
当唾液彻底干燥时，其污染对RMGIC与RC之间的μSBS没有影响，而血液和血液 - 唾液污染即使在彻底干燥时也会降低RMGIC与RC之间的μSBS。
