Wang Mengying, Dong Biying, Song Zhihua, Qi Meng, Chen Ting, Du Tingting, Cao Hongyan, Liu Ni, Meng Dong, Yang Qing, Fu Yujie
The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing, 100083, China; Ecological Observation and Research Station of Heilongjiang Sanjiang Plain Wetlands, National Forestry and Grassland Administration, Beijing Forestry University, Beijing, China.
Plant Physiol Biochem. 2023 Mar;196:381-392. doi: 10.1016/j.plaphy.2023.02.002. Epub 2023 Feb 3.
Flavonoids are important secondary metabolites in the plant growth and development process. As a medicinal plant, pigeon pea is rich in secondary metabolites. As a flavonoid, there are few studies on the regulation mechanism of naringenin in plant stress resistance. In our study, we found that naringenin can increase the pigeon pea's ability to tolerate salt and influence the changes that occur in flavonoids including naringenin, genistein and biochanin A. We analyzed the transcriptome data after 1 mM naringenin treatment, and identified a total of 13083 differentially expressed genes. By analyzing the metabolic pathways of these differentially expressed genes, we found that these differentially expressed genes were enriched in the metabolic pathways of phenylpropanoid biosynthesis, starch and sucrose metabolism and so on. We focused on the analysis of flavonoid biosynthesis related pathways. Among them, the expression levels of enzyme genes CcIFS, CcCHI and CcCHS in the flavonoid biosynthesis pathway had considerably higher expression levels. By counting the number of transcription factors and the binding sites on the promoter of the enzyme gene, we screened the transcription factors CcMYB62 and CcbHLH35 related to flavonoid metabolism. Among them, CcMYB62 has a higher expression level than the others. The hairy root transgene showed that CcMYB62 could induce the upregulation of CcCHI, and promote the accumulation of naringenin, genistein and biochanin A. Our study revealed the molecular mechanism of naringenin regulating flavonoid biosynthesis under salt stress in pigeon pea, and provided an idea for the role of flavonoids in plant resistance to abiotic stresses.
黄酮类化合物是植物生长发育过程中重要的次生代谢产物。作为一种药用植物，木豆富含次生代谢产物。作为一种黄酮类化合物，关于柚皮素在植物抗逆性调控机制方面的研究较少。在我们的研究中，我们发现柚皮素可以提高木豆的耐盐能力，并影响包括柚皮素、染料木黄酮和鹰嘴豆芽素A在内的黄酮类化合物的变化。我们分析了1 mM柚皮素处理后的转录组数据，共鉴定出13083个差异表达基因。通过分析这些差异表达基因的代谢途径，我们发现这些差异表达基因在苯丙烷生物合成、淀粉和蔗糖代谢等代谢途径中富集。我们重点分析了黄酮类生物合成相关途径。其中，黄酮类生物合成途径中的酶基因CcIFS、CcCHI和CcCHS的表达水平显著较高。通过统计转录因子的数量以及酶基因启动子上的结合位点，我们筛选出了与黄酮类代谢相关的转录因子CcMYB62和CcbHLH35。其中，CcMYB62的表达水平高于其他转录因子。毛状根转基因实验表明，CcMYB62可以诱导CcCHI的上调表达，并促进柚皮素、染料木黄酮和鹰嘴豆芽素A的积累。我们的研究揭示了柚皮素在盐胁迫下调控木豆黄酮类生物合成的分子机制，并为黄酮类化合物在植物抗非生物胁迫中的作用提供了思路。
