State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, PR China.
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, PR China; School of Civil Engineering, Lanzhou University of Technology, Lanzhou, 730070, PR China.
Environ Res. 2022 Sep;212(Pt D):113530. doi: 10.1016/j.envres.2022.113530. Epub 2022 May 21.
Distribution of intracellular (iARGs) and extracellular ARGs (eARGs) in manure anaerobic digestion (AD) process coupled with two types of biochar (BC and BP) were investigated. And the effects of biochar on the conjugation transfer of ARGs were explored by deciphering the interaction of biochar with bacterial stress responses, physiological metabolism and antibiotic resistances. Results showed that AD process could effectively remove all the detected eARGs with efficiency of 47.4-98.2%. The modified biochar (BP) with larger specific surface area (SSA) was propitious to decrease the absolute copy number of extracellular resistance genes. AD process could effectively remove iARGs by inhibiting the growth of host bacteria. The results of structural equation models (SEM) indicated that biochar put indirect influences on the fate of ARGs (λ = -0.23, P > 0.05). Analysis on oxidative stress levels, antioxidant capacity, DNA damage-induced response (SOS) response and energy generation process demonstrated that biochar induced the oxidative stress response of microorganisms and enhanced the antioxidant capacity of bacteria. The elevated antioxidant capacity negatively affected SOS response, amplified cell membrane damage and further weakened the energy generation process, resulted in the inhibition of horizontal transfer of ARGs.
研究了粪污厌氧消化（AD）过程中细胞内（iARGs）和细胞外 ARGs（eARGs）的分布情况，以及两种生物炭（BC 和 BP）对 ARGs 共转移的影响。通过解析生物炭与细菌应激反应、生理代谢和抗生素抗性的相互作用，探讨了生物炭对 ARGs 共转移的影响。结果表明，AD 过程可有效去除所有检测到的 eARGs，去除效率为 47.4-98.2%。比表面积（SSA）较大的改性生物炭（BP）有利于减少细胞外抗性基因的绝对拷贝数。AD 过程可通过抑制宿主细菌的生长有效去除 iARGs。结构方程模型（SEM）的结果表明，生物炭对 ARGs 的命运有间接影响（λ=−0.23，P＞0.05）。氧化应激水平、抗氧化能力、DNA 损伤诱导响应（SOS 响应）和能量产生过程的分析表明，生物炭诱导了微生物的氧化应激反应，增强了细菌的抗氧化能力。抗氧化能力的提高对 SOS 反应产生负面影响，放大了细胞膜损伤，进一步削弱了能量产生过程，从而抑制了 ARGs 的水平转移。
