Zhao Xingyue, Li Qian, Liu Wenjing, Guan Haiqing, Li Cheng, Wang Jihui, Wang Liang
School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
School of Life Sciences and Biotechnology, Dalian University, Dalian 116622, Liaoning, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Jul 25;36(7):1293-1304. doi: 10.13345/j.cjb.190500.
Cordycepin as the main active ingredient of Cordyceps militaris, a traditional medicinal fungus in China, has many physiological functions such as anti-cancer, anti-tumor and anti-virus activity. The most potential route for effective cordycepin production has been considered as liquid fermentation of C. militaris though with low productivity at present. Thus, it is urgent to apply both process engineering strategy and metabolic engineering strategy to enhance the productivity of cordycepin. In this review, the effects of medium components (i.e. the carbon/nitrogen source, precursor substances and metal ions) and operation factors (i.e. pH, dissolved oxygen and light) on cordycepin biosynthesis in liquid fermentation system are summarized. Besides, separation of cordycepin, the gene cluster involved and predicted biosynthesis pathways of cordycepin are also discussed, providing possible solutions of finally realizing efficient production of cordycepin.
虫草素是中国传统药用真菌蛹虫草的主要活性成分，具有许多生理功能，如抗癌、抗肿瘤和抗病毒活性。尽管目前蛹虫草液体发酵生产虫草素的效率较低，但这仍是生产虫草素最具潜力的途径。因此，迫切需要应用过程工程策略和代谢工程策略来提高虫草素的产量。在这篇综述中，总结了培养基成分（即碳源/氮源、前体物质和金属离子）和操作因素（即pH值、溶解氧和光照）对液体发酵系统中虫草素生物合成的影响。此外，还讨论了虫草素的分离、相关基因簇以及预测的虫草素生物合成途径，为最终实现虫草素的高效生产提供了可能的解决方案。
