Toepel Jörg, Karande Rohan, Bühler Bruno, Bühler Katja, Schmid Andreas
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.
Research and Transfer Center for bioactive Matter b-ACT(matter), University of Leipzig, Germany.
Bioresour Technol. 2023 Apr;373:128703. doi: 10.1016/j.biortech.2023.128703. Epub 2023 Feb 4.
Hydrogen (H) is a promising fuel in the context of climate neutral energy carriers and photosynthesis-driven H-production is an interesting option relying mainly on sunlight and water as resources. However, this approach depends on suitable biocatalysts and innovative photobioreactor designs to maximize cell performance and H titers. Cyanobacteria were used as biocatalysts in capillary biofilm photobioreactors (CBRs). We show that biofilm formation/stability depend on light and CO availabilityH production rates correlate with these parameters but differ between Anabaena and Nostoc. We demonstrate that high light and corresponding O levels influence biofilm stability in CBR. By adjusting these parameters, biofilm formation/stability could be enhanced, and H formation was stable for weeks. Final biocatalyst titers reached up to 100 g l for N. punctiforme atcc 29133 NHM5 and Anabaena sp. pcc 7120 AMC 414. H production rates were up to 300 µmol H lh and 3 µmol H gh in biofilms.
在气候中和能源载体的背景下，氢气（H）是一种很有前景的燃料，而光合作用驱动的产氢是一种主要依赖阳光和水作为资源的有趣选择。然而，这种方法依赖于合适的生物催化剂和创新的光生物反应器设计，以最大限度地提高细胞性能和氢气产量。蓝细菌被用作毛细管生物膜光生物反应器（CBRs）中的生物催化剂。我们表明生物膜的形成/稳定性取决于光照和二氧化碳的可用性，产氢速率与这些参数相关，但鱼腥藻和念珠藻之间存在差异。我们证明高光和相应的氧气水平会影响CBR中生物膜的稳定性。通过调整这些参数，可以增强生物膜的形成/稳定性，并且氢气的形成可以稳定数周。对于点状念珠藻atcc 29133 NHM5和鱼腥藻sp. pcc 7120 AMC 414，最终生物催化剂产量高达100 g l。生物膜中的产氢速率分别高达300 μmol H lh和3 μmol H gh。
