Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD, USA.
Composites and Polymer Engineering Laboratory, South Dakota School of Mines and Technology, Rapid City, SD, USA.
Biofouling. 2020 Feb;36(2):183-199. doi: 10.1080/08927014.2020.1748186. Epub 2020 Apr 13.
Although cyanobacteria are a common group of microorganisms well-suited to utilization in photobioreactors (PBRs), studies of cyanobacteria fouling and its prevention are scarce. Using a cyanobacterium, sp. PCC 7120, which had been genetically modified to enhance linalool production, the formation of conditioning films and the effects of these on the physico-chemical surface properties of various PBR materials during initial adhesion and biofilm formation were investigated. The adhesion assay revealed that the overall attachment of was substratum dependent and no correlation between the hydrophobicity/roughness of clean material and cell attachment was found. Surface hydrophilicity/hydrophobicity of all the materials changed within 12h due to formation of conditioning films. ATR-FTIR spectroscopy revealed that the fractional change in protein deposition between 12 to 96h was consistent with cell attachment but polysaccharide deposition was material specific and did not correlate with cell attachment on the PBR materials. Also, the delay in conditioning film proteins on PVC and PTFE indicated that components other than proteins may be responsible for the decrease in contact angles on these surfaces within 12h. This indicates the important role of the chemical nature of adsorbed conditioning films in determining the initial attachment of to PBR materials. The lower rate of attachment of on the hydrophilic surfaces (glass and PMMA) between 72h to 96h (regime 3) showed that these surfaces could potentially have low fouling characteristics at extended time scales and should be considered for further research.
尽管蓝藻是一类适合在光生物反应器（PBR）中利用的常见微生物，但对蓝藻结垢及其预防的研究却很少。本研究使用了一种经过基因改造以增强芳樟醇产量的蓝藻 sp. PCC 7120，研究了其形成的成膜过程以及这些成膜过程对各种 PBR 材料在初始附着和生物膜形成过程中物理化学表面性质的影响。附着实验表明， sp. 的总体附着与基底有关，且清洁材料的疏水性/粗糙度与细胞附着之间没有相关性。由于成膜过程的形成，所有材料的表面亲水性/疏水性在 12 小时内发生变化。ATR-FTIR 光谱分析表明，在 12 至 96 小时之间，蛋白质沉积的分数变化与 sp. 的细胞附着一致，但多糖沉积具有材料特异性，与 PBR 材料上细胞附着无关。此外，聚氯乙烯（PVC）和聚四氟乙烯（PTFE）上成膜蛋白的延迟表明，在这些表面的接触角在 12 小时内降低，可能不仅仅是由于蛋白质成分造成的。这表明吸附成膜的化学性质在决定 sp. 对 PBR 材料的初始附着中起着重要作用。在 72 小时至 96 小时（第 3 阶段）， sp. 在亲水表面（玻璃和 PMMA）上的附着率较低，这表明这些表面在较长时间尺度上可能具有低结垢特性，应进一步研究。
