College of Pharmacy, Xinjiang Medical University, No. 393 Xinyi Road, Urumqi, 830011, China.
The First Affiliated Hospital Of Xinjiang Medical University, No. 137 South Liyushan Road, Urumqi, 830054, China.
Sci Rep. 2018 Dec 10;8(1):17753. doi: 10.1038/s41598-018-36026-7.
Salvia deserta Schang (SDS) belongs to the same family as Salvia miltiorrhiza bunge, one of the antithrombotic Chinese herbal medicines. In our study, EtOAc root extracts were analyzed for their effects on adenosine diphosphate (ADP)-induced platelet aggregation in rabbits and FeCl-induced rat common carotid artery thrombosis as well as on rat blood plasma concentrations of thromboxane B2 (TXB), 6-keto-prostaglandin F1 alpha (6-keto-PGF), antithrombin-III (AT-III), protein C (PC), plasminogen (PLG), plasminogen activator inhibitor (PAI-1), von Willebrand factor (vWF) and tissue-type plasminogen activator (t-PA). EtOAc extracts from SDS roots had significant inhibitory effects on ADP-induced maximum platelet aggregation rate (10.2 ± 2.6 vs control 35.7 ± 5.2; P < 0.05), reduced the FeCl-induced rat common carotid artery thrombus weight and thrombus area ratio (P < 0.05), significantly decreased plasma TXB, vWF and PAI-1 levels and increased 6-keto-PGF and t-PA levels in a dose dependent manner (all P < 0.05). Thus, the ratio of TXB/6-keto-PGF was significantly decreased (P < 0.05), while the ratio of t-PA/PAI-1 was significantly increased (P < 0.05). In addition, enhanced AT-III and PC activities indicated coagulation inactivation effects of EtOAc SDS root extracts. EtOAc extraction from SDS showed antithrombotic effects, which are likely due to platelet adhesion and aggregation inhibition as well as anticoagulant activities.
荒漠鼠尾草（SDS）属于丹参的同一科，丹参是一种抗血栓形成的中草药。在我们的研究中，分析了 SDS 根的乙酸乙酯提取物对兔血小板聚集诱导剂二磷酸腺苷（ADP）的影响，以及对 FeCl3 诱导的大鼠颈总动脉血栓形成的影响，同时还分析了 SDS 根的乙酸乙酯提取物对大鼠血浆血栓素 B2（TXB）、6-酮-前列腺素 F1α（6-keto-PGF）、抗凝血酶-III（AT-III）、蛋白 C（PC）、纤溶酶原（PLG）、纤溶酶原激活物抑制剂（PAI-1）、血管性血友病因子（vWF）和组织型纤溶酶原激活物（t-PA）的影响。SDS 根的乙酸乙酯提取物对 ADP 诱导的最大血小板聚集率有显著的抑制作用（10.2±2.6 vs 对照组 35.7±5.2；P<0.05），降低了 FeCl3 诱导的大鼠颈总动脉血栓重量和血栓面积比（P<0.05），显著降低了血浆 TXB、vWF 和 PAI-1 水平，并呈剂量依赖性增加了 6-keto-PGF 和 t-PA 水平（均 P<0.05）。因此，TXB/6-keto-PGF 的比值显著降低（P<0.05），而 t-PA/PAI-1 的比值显著增加（P<0.05）。此外，增强的 AT-III 和 PC 活性表明 SDS 根乙酸乙酯提取物具有凝血失活作用。SDS 的乙酸乙酯提取物表现出抗血栓作用，这可能是由于抑制血小板黏附和聚集以及抗凝活性。
