SubramanianBalachandar V A, Steward R L
University of Central Florida.
Res Sq. 2023 Jan 27:rs.3.rs-2499973. doi: 10.21203/rs.3.rs-2499973/v1.
ECM composition is important in a host of pathophysiological processes such as angiogenesis, atherosclerosis, and diabetes, for example and during each of these processes ECM composition has been reported to change over time. However, the impact ECM composition has on the endothelium’s ability to respond mechanically is currently unknown. Therefore, in this study we seeded human umbilical vein endothelial cells (HUVECs) onto soft hydrogels coated with an ECM concentration of 0.1 mg/mL at the following collagen I (Col-I) and fibronectin (FN) ratios: 100%Col-I, 75%Col-I-25%FN, 50%Col-I-50%FN, 25%Col-I-75%FN, and 100%FN. We subsequently measured tractions, intercellular stresses, strain energy, cell morphology, and cell velocity. Our results revealed huvecs seeded on gels coated with 50% Col-I - 50% FN to have the highest intercellular stresses, tractions, strain energies, but the lowest velocities and cell circularity. Huvecs seeded on 100% Col-I had the lowest tractions, cell area while havingthe highest velocities and cell circularity. In addition, cells cultured on 25% Col-I and 75% FN had the lowest intercellular stresses, but the highest cell area. Huvecs cultured on 100% FN yielded the lowest strain energies. We believe these results will be of great importance to the cardiovascular field, biomedical field, and cell mechanics. Study the influence of different Col-I - FN ECM compositions on endothelial cell mechanics and morphology.
例如，细胞外基质（ECM）的组成在许多病理生理过程中都很重要，如血管生成、动脉粥样硬化和糖尿病，并且在这些过程中的每一个过程中，据报道ECM组成都会随时间变化。然而，ECM组成对内皮细胞机械反应能力的影响目前尚不清楚。因此，在本研究中，我们将人脐静脉内皮细胞（HUVECs）接种到涂有浓度为0.1 mg/mL ECM的软水凝胶上，其胶原蛋白I（Col-I）和纤连蛋白（FN）的比例如下：100%Col-I、75%Col-I-25%FN、50%Col-I-50%FN、25%Col-I-75%FN和100%FN。随后，我们测量了牵引力、细胞间应力、应变能、细胞形态和细胞速度。我们的结果显示，接种在涂有50%Col-I - 50%FN凝胶上的HUVECs具有最高的细胞间应力、牵引力、应变能，但速度和细胞圆形度最低。接种在100%Col-I上的HUVECs牵引力和细胞面积最低，而速度和细胞圆形度最高。此外，在25%Col-I和75%FN上培养的细胞细胞间应力最低，但细胞面积最大。在100%FN上培养的HUVECs产生的应变能最低。我们相信这些结果将对心血管领域、生物医学领域和细胞力学具有重要意义。研究不同Col-I - FN ECM组成对内皮细胞力学和形态的影响。
