Madeddu Manuela, Zaniboni Luisa, Marelli Stefano Paolo, Comazzi Stefano, Cerolini Silvia
Department of Veterinary Medicine and Animal Science, University of Milan, via dell'Università 6, Lodi 26900, Italy.
Data Brief. 2023 Jan 19;47:108916. doi: 10.1016/j.dib.2023.108916. eCollection 2023 Apr.
This article includes supporting data regarding the research article entitled "Concentration dependent effect of dimethylacetamide and N-methylacetamide on the quality and fertility of cryopreserved chicken semen" (Zaniboni et al., 2022). The effect of two permeant-cryoprotectants (CPA), dimethylacetamide (DMA) and N-methylacetamide (NMA) used at different concentrations (0, 2, 4 and 6%) on the quality of post thaw rooster semen was assessed. Ejaculates were processed according to 7 treatments: Lake pre-freezing+0.1 M trehalose (LPF-T) (control treatment), LPF-T+2% DMA (DMA2), LPF-T+4% DMA (DMA4), LPF-T+6% DMA (DMA6), LPF-T+2% NMA (NMA2), LPF-T+4% NMA (NMA4), LPF-T+6% NMA (NMA6). Sperm acrosome integrity and mitochondrial activity were investigated in frozen-thawed semen with the use of the flow cytometry technique. Only the mitochondrial activity was significantly affected by the different cryoprotectant concentrations.
本文包含了与题为《二甲基乙酰胺和N-甲基乙酰胺对冷冻保存鸡精液质量和受精能力的浓度依赖性影响》（扎尼博尼等人，2022年）的研究文章相关的支持数据。评估了两种渗透性冷冻保护剂（CPA），即不同浓度（0%、2%、4%和6%）的二甲基乙酰胺（DMA）和N-甲基乙酰胺（NMA）对解冻后公鸡精液质量的影响。根据7种处理方法对射精进行处理：莱克预冻+0.1M海藻糖（LPF-T）（对照处理）、LPF-T+2% DMA（DMA2）、LPF-T+4% DMA（DMA4）、LPF-T+6% DMA（DMA6）、LPF-T+2% NMA（NMA2）、LPF-T+4% NMA（NMA4）、LPF-T+6% NMA（NMA6）。使用流式细胞术技术对冻融精液中的精子顶体完整性和线粒体活性进行了研究。只有线粒体活性受到不同冷冻保护剂浓度的显著影响。
