Colavita Francesca, Mazzotta Valentina, Rozera Gabriella, Abbate Isabella, Carletti Fabrizio, Pinnetti Carmela, Matusali Giulia, Meschi Silvia, Mondi Annalisa, Lapa Daniele, Vita Serena, Minosse Claudia, Aguglia Camilla, Gagliardini Roberta, Specchiarello Eliana, Bettini Aurora, Nicastri Emanuele, Girardi Enrico, Vaia Francesco, Antinori Andrea, Maggi Fabrizio
Laboratory of Virology, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, Italy.
Clinical and Research Infectious Diseases Department, National Institute for Infectious Diseases, Lazzaro Spallanzani IRCCS, Rome 00149, Italy.
iScience. 2023 Mar 17;26(3):106102. doi: 10.1016/j.isci.2023.106102. Epub 2023 Feb 1.
We report the follow-up laboratory investigation of three MPXV cases infected in May-June 2022 from diagnosis to disease resolution, monitoring viral shedding in different body fluids and antibody kinetics. Out of 138 non-lesion samples, viral DNA was found in 92.3% saliva, 85.7% semen, 86.2% oropharyngeal swabs, 51.7% plasma, 46.1% stool, and 9.5% urine samples. Viral load quantified by digital PCR widely varied, but tend to be higher in oropharyngeal swabs, saliva, and stool. Replication competent virus was recovered from four out of seventeen samples, including 1 saliva, 1 oropharyngeal swabs, 1 semen, and 1 stool. The analysis of the antibody kinetics revealed that IgM, IgA, and IgG antibodies were detected within two weeks post-symptoms onset for all three patients, with IgG detected early on at day 4-8 and IgM and IgA showing lower titers along the time frame of the study. Antibody levels increased during the second week of illness with IgG reaching high titers.
我们报告了对2022年5月至6月感染的3例猴痘病例从诊断到疾病痊愈的后续实验室调查情况，监测了不同体液中的病毒脱落情况和抗体动力学。在138份非病变样本中，病毒DNA在以下样本中被检测到：92.3%的唾液、85.7%的精液、86.2%的口咽拭子、51.7%的血浆、46.1%的粪便以及9.5%的尿液样本。通过数字PCR定量的病毒载量差异很大，但在口咽拭子、唾液和粪便中往往较高。在17份样本中的4份中分离出了具有复制能力的病毒，包括1份唾液、1份口咽拭子、1份精液和1份粪便。抗体动力学分析显示，所有3例患者在症状出现后两周内均检测到IgM、IgA和IgG抗体，IgG在第4 - 8天早期被检测到，IgM和IgA在研究时间段内滴度较低。在患病的第二周抗体水平升高，IgG达到高滴度。
