Choi Ji Young, Park Jam-Eon, Choi Seung-Hyeon, Kim Ji-Sun, Lee Jung-Sook, Lee Ju Huck, Kim Hyeun Bum, Lee Ju-Hoon, Kim Jae-Kyung, Kang Se Won, Park Seung-Hwan
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
Department of Animal Resources Science, Dankook University, Cheonan 31116, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005631.
A Gram-stain-negative, obligately anaerobic, non-motile, non-spore-forming, helical rod-shaped bacterium, designated AGMB01872, was isolated from faeces of a cow deposited in the National Institute of Animal Science (Wanju, Republic of Korea). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain AGMB01872 was most closely related to DSM 3072 (= KCTC 25222, 96.6 %) which belonged to the family . Growth was occurred at 30-40 °C (optimum, 37 °C), pH 6-7 (optimum, pH 7) and in the presence of 0.5-1.0 % (w/v) NaCl. The genomic DNA G+C content of strain AGMB01872 was 35.9 mol%. The average nucleotide identity value between strain AGMB01872 and DSM 3072 was 72.1 %. Cells of strain AGMB01872 utilized d-glucose, maltose, d-xylose and l-arabinose. The major fatty acids (>10 %) were C (23.9 %), C (29.4 %), summed feature 5 (10.8 %) and summed feature 10 (30.3 %). The major end-product of glucose fermentation was succinate. Based on the phenotypic, phylogenetic, biochemical, genotypic and chemotaxonomic data, AGMB01872 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is AGMB01872 (= KCTC 25201=NBRC 115007=GDMCC 1.2573).
从韩国国立动物科学研究所(完州)一头奶牛的粪便中分离出一株革兰氏染色阴性、专性厌氧、无运动性、不产芽孢、螺旋状杆状细菌,命名为AGMB01872。基于16S rRNA基因序列的系统发育分析表明,菌株AGMB01872与属于该科的DSM 3072(=KCTC 25222,96.6%)关系最为密切。在30-40°C(最适温度37°C)、pH 6-7(最适pH 7)以及0.5-1.0%(w/v)NaCl存在的条件下生长。菌株AGMB01872的基因组DNA G+C含量为35.9 mol%。菌株AGMB01872与DSM 3072之间的平均核苷酸同一性值为72.1%。菌株AGMB01872的细胞利用d-葡萄糖、麦芽糖、d-木糖和l-阿拉伯糖。主要脂肪酸(>10%)为C(23.9%)、C(29.4%)、总和特征5(10.8%)和总和特征10(30.3%)。葡萄糖发酵的主要终产物是琥珀酸。基于表型、系统发育、生化、基因型和化学分类学数据,AGMB01872代表该属内的一个新物种,为此提出新物种名称sp. nov.。模式菌株为AGMB01872(=KCTC 25201=NBRC 115007=GDMCC 1.2573)。