gen. nov., sp. nov., a novel member of the family isolated from pit clay used for making Chinese strong aroma-type liquor.

An anaerobic, Gram-stain-positive, rod-shaped, motile and spore-forming bacterium, designated strain LBM18003, was isolated from pit clay used for making Chinese strong aroma-type liquor. Growth occurred at 20-40 °C (optimum, 30-37 °C), pH 4.5-9.5 (optimum, pH 6.5-7.0) and in the presence of 0.0-1.0 % (w/v) sodium chloride (optimum, 0 %). The predominant fatty acids were C, C, C DMA and C 3-OH, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and nine unidentified glycolipids. Phylogenetic analysis revealed that strain LBM18003 is a novel member of the family . The 16S rRNA gene sequence similarities of strain LBM18003 to its two most closely related species were less than 94.5 % for distinguishing genera, i.e. closely related to JCM 30532 (94.1 %) and DSM 107079 (93.2 %). The genome size of strain LBM18003 was 2 996 201 bp and its DNA G+C content was 48.48 mol%. Strain LBM18003 exhibited 67.8 and 68.1% pairwise-determined whole-genome average nucleotide identity values to JCM 30532 and DSM 107079, respectively; and showed 62.2 and 61.0 % the average amino acid identity values to JCM 30532 and DSM 107079, respectively; and demonstrated 46.1 and 41.5 % conserved genes to JCM 30532 and DSM 107079, respectively. The comparisons of 16S rRNA gene and genome sequences confirmed that strain LBM18003 represented a novel genus of the family . Based on morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic results, strain LBM18003 represents a novel species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is LBM18003 (=GDMCC 1.1626=JCM 33783).