Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
National Engineering Research Center of Solid-state Brewing, Luzhou 646000, PR China.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004789.
An anaerobic, Gram-stain-positive, rod-shaped, motile and spore-forming bacterium, designated strain LBM18003, was isolated from pit clay used for making Chinese strong aroma-type liquor. Growth occurred at 20-40 °C (optimum, 30-37 °C), pH 4.5-9.5 (optimum, pH 6.5-7.0) and in the presence of 0.0-1.0 % (w/v) sodium chloride (optimum, 0 %). The predominant fatty acids were C, C, C DMA and C 3-OH, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and nine unidentified glycolipids. Phylogenetic analysis revealed that strain LBM18003 is a novel member of the family . The 16S rRNA gene sequence similarities of strain LBM18003 to its two most closely related species were less than 94.5 % for distinguishing genera, i.e. closely related to JCM 30532 (94.1 %) and DSM 107079 (93.2 %). The genome size of strain LBM18003 was 2 996 201 bp and its DNA G+C content was 48.48 mol%. Strain LBM18003 exhibited 67.8 and 68.1% pairwise-determined whole-genome average nucleotide identity values to JCM 30532 and DSM 107079, respectively; and showed 62.2 and 61.0 % the average amino acid identity values to JCM 30532 and DSM 107079, respectively; and demonstrated 46.1 and 41.5 % conserved genes to JCM 30532 and DSM 107079, respectively. The comparisons of 16S rRNA gene and genome sequences confirmed that strain LBM18003 represented a novel genus of the family . Based on morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic results, strain LBM18003 represents a novel species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is LBM18003 (=GDMCC 1.1626=JCM 33783).
一株厌氧、革兰氏阳性、杆状、运动和孢子形成的细菌，被命名为 LBM18003 菌株，从用于制作中国浓香型白酒的窖泥中分离得到。生长温度为 20-40°C（最佳温度为 30-37°C），pH 值为 4.5-9.5（最佳 pH 值为 6.5-7.0），并在 0.0-1.0%（w/v）氯化钠（最佳 0%）存在的情况下生长。主要脂肪酸为 C、C、C DMA 和 C 3-OH，主要极性脂类为双磷脂酰甘油、磷脂酰甘油、两种未鉴定的磷脂和九种未鉴定的糖脂。系统发育分析表明，LBM18003 菌株是 科的一个新成员。LBM18003 菌株与两个最密切相关的种的 16S rRNA 基因序列相似性小于 94.5%，用于区分属，即与 JCM 30532（94.1%）和 DSM 107079（93.2%）密切相关。LBM18003 菌株的基因组大小为 2996201bp，其 DNA G+C 含量为 48.48mol%。LBM18003 菌株与 JCM 30532 和 DSM 107079 的全基因组平均核苷酸同一性值分别为 67.8%和 68.1%；与 JCM 30532 和 DSM 107079 的平均氨基酸同一性值分别为 62.2%和 61.0%；与 JCM 30532 和 DSM 107079 的保守基因分别为 46.1%和 41.5%。16S rRNA 基因和基因组序列的比较证实，LBM18003 菌株代表了 科的一个新属。基于形态学、生理学、生物化学、化学分类学、基因型和系统发育结果，LBM18003 菌株代表了 科的一个新种，属名暂定为 gen. nov.，种名暂定为 sp. nov.。模式菌株为 LBM18003（=GDMCC 1.1626=JCM 33783）。
