Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-Gu, Seongnam-si, Gyeonggi-do 13620, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Mar;70(3):2059-2065. doi: 10.1099/ijsem.0.004015.
An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111, was isolated from faeces from a healthy Korean. Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111 was closely related to CCRI-16110 (93.9 %) and GAM6-1 (93.7 %), followed by DSM 1787 (93.5 %), ATCC 27340 (93.4 %), DSM 10507 (93.1 %) and ATCC 29236 (93.1 %) within the family ( rRNA cluster XIVa). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111 formed a separate branch with species in the genus . The major cellular fatty acids (>10.0 %) were C and C 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids. KGMB01111 contained diaminopimelic acid in cell-wall peptidoglycan. The predominant end product of fermentation produced by KGMB01111 was acetic acid. Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111 represents a novel species within the genus for which the name sp. nov. is proposed. The type strain is KGMB01111 (=KCTC 15706=DSM 107827).
一株严格厌氧、革兰氏阳性、非运动、球形或卵圆形的细菌，被命名为 KGMB01111 菌株，从一名健康韩国人的粪便中分离得到。16S rRNA 基因序列的比较分析表明，KGMB01111 与 CCRI-16110（93.9%）和 GAM6-1（93.7%）最为密切相关，其次是 DSM 1787（93.5%）、ATCC 27340（93.4%）、DSM 10507（93.1%）和 ATCC 29236（93.1%），都属于 （rRNA 聚类 XIVa）家族。基于 16S rRNA 基因序列的系统发育分析表明，KGMB01111 与 属内的种形成了一个单独的分支。主要的细胞脂肪酸（>10.0%）为 C 和 C 9 二甲基缩醛（DMA），主要的极性脂类为胺磷脂和脂类。KGMB01111 的细胞壁肽聚糖中含有二氨基庚二酸。KGMB01111 发酵的主要终产物是乙酸。根据全基因组序列，该分离株的 DNA G+C 含量为 44.7mol%。基于表型、化学分类和系统发育特征，KGMB01111 代表了 属中的一个新种，建议将其命名为 sp. nov.。模式菌株为 KGMB01111（=KCTC 15706=DSM 107827）。
