Kim Do-Yeon, Jeong In-Chul, Lee So-Yeon, Jeong Yun-Seok, Han Jeong Eun, Tak Euon Jung, Lee June-Young, Kim Pil Soo, Hyun Dong-Wook, Bae Jin-Woo
Department of Biology and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul 02447, Republic of Korea.
Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005643.
Two novel Gram-stain-positive, non-motile and non-spore-forming bacterial strains, designated J2M5 and J1M15, were isolated from the gastrointestinal tract of a lake prawn . Strain J2M5 was an obligately aerobic bacterium that formed milky-coloured colonies and showed a rod-coccus cell cycle, while strain J1M15 was a facultatively aerobic bacterium that formed orangish-yellow-coloured colonies and showed rod-shaped cells. Strains J2M5 and J1M15 showed the highest 16S rRNA gene sequence similarity to JC2055 (98.63 %) and SST-39 (98.08 %), respectively. The whole-genome sequence of strain J2M5 was 4.52 Mbp in size and the genomic G+C content directly calculated from the genome sequence of strain J2M5 was 72.5 mol%. The whole-genome sequence of strain J1M15 was 3.20 Mbp in size and the genomic G+C content directly calculated from the genome sequence of strain J1M15 was 69.6mol %. Strains J2M5 and J1M15 showed high OrthoANI similarity to JC2055 (83.6 %) and (77.2 %), respectively. We analysed the genome sequences of strains J2M5 and J1M15 in terms of carbohydrate-active enzymes, antibiotic resistance genes and virulence factor genes. Strains J2M5 and J1M15 contained MK-8 (H) and MK-9 (H) as the predominant respiratory quinones, respectively. The major polar lipids of both strains were phosphatidylglycerol and diphosphatidylglycerol. Additionally, strain J2M5 possessed phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine. The cellular sugar components of strain J2M5 were ribose, mannose, glucose and galactose, and its cellular amino acid components were l-alanine and l-lysine. The cellular sugar components of strain J1M15 were rhamnose, ribose, mannose and glucose, and its cellular amino acid component was l-alanine. The major cellular fatty acids of strains J2M5 and J1M15 were iso-C and anteiso-C, respectively. The multiple taxonomic analyses indicated that strains J2M5 and J1M15 represent novel species of the genus and , respectively. We propose the names sp. nov. and sp. nov. for strain J2M5 (=KCTC 49461=CCUG 74767) and strain J1M15 (=KCTC 49462=CCUG 74766), respectively.
从一只湖虾的胃肠道中分离出两株新的革兰氏染色阳性、无运动性且不形成芽孢的细菌菌株，分别命名为J2M5和J1M15。菌株J2M5是专性需氧菌，形成乳白色菌落，呈现杆状 - 球菌细胞周期，而菌株J1M15是兼性需氧菌，形成橙黄色菌落，呈现杆状细胞。菌株J2M5和J1M15的16S rRNA基因序列与JC2055（98.63%）和SST - 39（98.08%）的相似性最高。菌株J2M5的全基因组序列大小为4.52 Mbp，直接从菌株J2M5的基因组序列计算得到的基因组G + C含量为72.5 mol%。菌株J1M15的全基因组序列大小为3.20 Mbp，直接从菌株J1M15的基因组序列计算得到的基因组G + C含量为69.6 mol%。菌株J2M5和J1M15与JC2055（83.6%）和（77.2%）分别显示出较高的OrthoANI相似性。我们从碳水化合物活性酶、抗生素抗性基因和毒力因子基因方面分析了菌株J2M5和J1M15的基因组序列。菌株J2M5和J1M15分别以MK - 8（H）和MK - 9（H）作为主要呼吸醌。两株菌株的主要极性脂类都是磷脂酰甘油和二磷脂酰甘油。此外，菌株J2M5还拥有磷脂酰胆碱、磷脂酰丝氨酸和磷脂酰乙醇胺。菌株J2M5的细胞糖成分是核糖、甘露糖、葡萄糖和半乳糖，其细胞氨基酸成分是L - 丙氨酸和L - 赖氨酸。菌株J1M15的细胞糖成分是鼠李糖、核糖、甘露糖和葡萄糖，其细胞氨基酸成分是L - 丙氨酸。菌株J2M5和J1M15的主要细胞脂肪酸分别是异C和anteiso - C。多种分类学分析表明，菌株J2M5和J1M15分别代表属的新物种。我们分别为菌株J2M5（=KCTC 49461 = CCUG 74767）和菌株J1M15（=KCTC 49462 = CCUG 74766）提议命名为sp. nov.和sp. nov.。
