Sakdapetsiri Chatsuda, Ngaemthao Wipaporn, Suriyachadkun Chanwit, Pinyakong Onruthai
Center of Excellence in Microbial Technology for Marine Pollution Treatment (MiTMaPT), Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Plant Pathology, Faculty of Agriculture at Kamphaengsaen, Kasetsart University Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005651.
A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area. According to the results of a polyphasic taxonomic study, a novel species belonging to the genus was described. Strain ABSL32-1 grew optimally at 20-25 °C and at pH 6.0-8.0 in the presence of 1 % (w/v) NaCl. The whole-cell sugars were ribose, mannose and glucose. The peptidoglycan structure contained A4α peptidoglycan (Lys-Glu; A11.54). The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids. The major menaquinones were MK-9 and MK-10. The major cellular fatty acid was anteiso-C (70.1 %). Based on 16S rRNA gene sequence analysis, strain ABSL32-1 showed the highest similarity to DSM 20167 (99.5 %), followed by SPC26 (99.0 %) and AG31 (98.8 %). The genome of strain ABSL32-1 is 4.4 Mbp with a DNA G+C content of 66.0 mol%. The average nucleotide identity values between strain ABSL32-1 and the type strains DSM20167, SPC26 and AG31 were 86.6, 74.7 and 83.6 %, respectively. On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1 is proposed to represent a novel species to be named sp. nov. The type strain is ABSL32-1 (=TBRC 14976=NBRC 115252).
从素攀武里城市固体废弃物处理区采集的土壤样本中分离出一株革兰氏阳性、不规则短杆状且无运动性的细菌，命名为ABSL32 - 1菌株。根据多相分类研究结果，描述了一个属于该属的新物种。ABSL32 - 1菌株在20 - 25 °C以及pH 6.0 - 8.0、1 %（w/v）NaCl存在的条件下生长最佳。全细胞糖为核糖、甘露糖和葡萄糖。肽聚糖结构包含A4α肽聚糖（Lys - Glu；A11.54）。极性脂质包含二半乳糖基二酰基甘油、双磷脂酰甘油、磷脂酰甘油、未鉴定的磷脂和两种未鉴定的脂质。主要甲基萘醌为MK - 9和MK - 10。主要细胞脂肪酸为anteiso - C（70.1 %）。基于16S rRNA基因序列分析，ABSL32 - 1菌株与DSM 20167（99.5 %）相似度最高，其次是SPC26（99.0 %）和AG31（98.8 %）。ABSL32 - 1菌株的基因组为4.4 Mbp，DNA G + C含量为66.0 mol%。ABSL32 - 1菌株与模式菌株DSM20167、SPC26和AG31之间的平均核苷酸同一性值分别为86.6、74.7和83.6 %。基于表型、化学分类和基因型特征，提议ABSL32 - 1菌株代表一个新物种，命名为sp. nov.。模式菌株为ABSL32 - 1（=TBRC 14976=NBRC 115252）。
