Sakdapetsiri Chatsuda, Kuntaveesuk Aunchisa, Ngaemthao Wipaporn, Suriyachadkun Chanwit, Muangchinda Chanokporn, Chavanich Suchana, Viyakarn Voranop, Chen Bo, Pinyakong Onruthai
Microbial Technology for Marine Pollution Treatment Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Microbial Diversity and Utilization Research Team, Thailand Bioresource Research Center, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004689. Epub 2021 Feb 8.
A novel bacterium, designated strain ANT13_2, was isolated from a phenanthrene-degrading consortium enriched from a soil sample collected near the Great Wall Station located in the southwestern area of King George Island, Antarctica. Following a polyphasic taxonomic study, a novel species belonging to the genus was described. The strain was a Gram-stain-positive bacterium that exhibited a rod-coccus growth cycle. Strain ANT13_2 grew aerobically at an optimum temperature of 20-25 °C and at pH 7.0-8.0. Ribose, arabinose and glucose were detected as whole-cell sugars. The predominant menaquinone was MK-9. The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid. The predominant cellular fatty acids were anteiso-C (67.7 %) and anteiso-C (11.2 %). The DNA G+C content of the genomic DNA was 60.6 mol%. Based on 16S rRNA gene sequence analysis, strain ANT13_2 showed the highest similarities to SPC26 (98.9 %) followed by Lz1y (98.4 %), DSM 20167 (98.3%) and KGN15 (97.9 %). The average nucleotide identity values between strain ANT13_2 and the type strains of SPC26 and Lz1y were 73.8 and 77.5 %, respectively, which are well below the 95-96 % species circumscription threshold. On the basis of this polyphasic taxonomic study, strain ANT13_2 is proposed to represent a novel species to be named sp. nov. The type strain is ANT13_2 (=TBRC 11756=NBRC 114615).
从南极乔治王岛西南部长城站附近采集的土壤样本中富集得到的菲降解菌群中分离出一株新细菌，命名为ANT13_2菌株。经过多相分类研究，描述了一个属于该属的新物种。该菌株为革兰氏阳性菌，呈现杆状-球菌生长周期。ANT13_2菌株在20-25 °C的最适温度和pH 7.0-8.0条件下好氧生长。核糖、阿拉伯糖和葡萄糖被检测为全细胞糖。主要的甲基萘醌是MK-9。诊断性磷脂为双磷脂酰甘油、磷脂酰甘油和一种未鉴定的磷脂。主要的细胞脂肪酸是anteiso-C（67.7%）和anteiso-C（11.2%）。基因组DNA的G+C含量为60.6 mol%。基于16S rRNA基因序列分析，ANT13_2菌株与SPC26菌株（98.9%）相似度最高，其次是Lz1y菌株（98.4%）、DSM 20167菌株（98.3%）和KGN15菌株（97.9%）。ANT13_2菌株与SPC26菌株和Lz1y菌株的模式菌株之间的平均核苷酸同一性值分别为73.8%和77.5%，远低于95-96%的物种界定阈值。基于此多相分类研究，提议ANT13_2菌株代表一个新物种，命名为新种。模式菌株为ANT13_2（=TBRC 11756=NBRC 114615）。
